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Journal Articles eLife Year : 2016

Sequestration of host metabolism by an intracellular pathogen

Abstract

For intracellular pathogens, residence in a vacuole provides a shelter against cytosolic host defense to the cost of limited access to nutrients. The human pathogen Chlamydia trachomatis grows in a glycogen-rich vacuole. How this large polymer accumulates there is unknown. We reveal that host glycogen stores shift to the vacuole through two pathways: bulk uptake from the cytoplasmic pool, and de novo synthesis. We provide evidence that bacterial glycogen metabolism enzymes are secreted into the vacuole lumen through type 3 secretion. Our data bring strong support to the following scenario: bacteria co-opt the host transporter SLC35D2 to import UDP-glucose into the vacuole, where it serves as substrate for de novo glycogen synthesis, through a remarkable adaptation of the bacterial glycogen synthase. Based on these findings we propose that parasitophorous vacuoles not only offer protection but also provide a microorganism-controlled metabolically active compartment essential for redirecting host resources to the pathogens.
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pasteur-01397781 , version 1 (16-11-2016)

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Lena Gehre, Olivier Gorgette, Stéphanie Perrinet, Marie-Christine Prevost, Mathieu Ducatez, et al.. Sequestration of host metabolism by an intracellular pathogen. eLife, 2016, 5, pp.e12552. ⟨10.7554/eLife.12552⟩. ⟨pasteur-01397781⟩
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