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Journal Articles Immunity Year : 2014

Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.

1 Immunobiologie des Cellules dendritiques
2 CRT - Centre de Recherche Translationnelle - Center for Translational Science
3 CIH - Centre d'Immunologie Humaine
4 Substances Lichéniques et Photoprotection
5 CIMI - Centre d'Immunologie et de Maladies Infectieuses
6 Immunobiologie des Cellules Dendritiques
7 Myriad rules based medicine
8 Chambre Régionale d'Agriculture des Pays de la Loire
9 BGPB - Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall
10 LGP - Laboratoire de géographie physique : Environnements Quaternaires et Actuels
11 Département d'Immunologie - Department of Immunology
12 Immunorégulation
13 Génétique Evolutive Humaine - Human Evolutionary Genetics
14 Laboratory of Cellular Physiology and Immunology and Chris Browne Center
15 St. Giles Laboratory of Human Genetics of Infectious Diseases
16 Laboratory of Human Genetics of Infectious Diseases
17 IMAGINE - U1163 - Imagine - Institut des maladies génétiques
18 Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
19 Dynamiques des Réponses Immunes
20 Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4) - Lymphopoïèse
21 Cellule Pasteur
22 CIML - Centre d'Immunologie de Marseille - Luminy
23 Immunobiologie de l'Infection - Immunobiology of Infection
24 Département de Génomes et Génétique - Department of Genomes and Genetics
25 Mycologie moléculaire
26 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire
27 Microenvironnement et Immunité - Microenvironment and Immunity
28 Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions
29 CRESS - U1153 - Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle
30 IFMK Brest - Institut de formation en masso-kinésithérapie
31 Réponse humorale aux pathogènes
32 Signalisation des Cytokines - Cytokine Signaling
33 Service d'hépatologie médicale [CHU Cochin]
34 Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases
35 CERLIS - UMR 8070 - Centre de recherche sur les liens sociaux
36 Biologie systémique - Systems Biology
37 CITECH - Imagopole
38 Laboratoire d'Immunologie Clinique
39 Génomique virale et vaccination
40 U753 - Cytokines et Immunologie des Tumeurs Humaines
41 Hôpital Saint-Louis
42 ICAReB - Investigation Clinique et d’Accès aux Ressources Biologiques (Plate-forme) - Clinical Investigation and Access to BioResources
Darragh Duffy
Milena Hasan
Philippe Bousso
James Di santo
  • Function : Author
Antonio Freitas
  • Function : Author
Ivo Gomperts-Boneca
  • Function : Author
Olivier Lantz
Hugo Mouquet
Olivier Schwartz
  • Function : Author
  • PersonId : 999827
Spencer Shorte
  • Function : Author
Vassili Soumelis
Frédéric Tangy
Eric Tartour
Antoine Toubert

Abstract

Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.

Domains

Immunology

Dates and versions

pasteur-01384537 , version 1 (20-10-2016)

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Cite

Darragh Duffy, Vincent Rouilly, Valentina Libri, Milena Hasan, Benoit Beitz, et al.. Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.. Immunity, 2014, 40 (3), pp.436-50. ⟨10.1016/j.immuni.2014.03.002⟩. ⟨pasteur-01384537⟩
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