Low SAMHD1 expression following T-cell activation and proliferation renders CD4+ T cells susceptible to HIV-1. - Institut Pasteur Access content directly
Journal Articles AIDS. Official journal of the international AIDS Society Year : 2015

Low SAMHD1 expression following T-cell activation and proliferation renders CD4+ T cells susceptible to HIV-1.

Olivier Schwartz
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Abstract

HIV-1 replication depends on the state of cell activation and division. It is established that SAMHD1 restricts HIV-1 infection of resting CD4 T cells. The modulation of SAMHD1 expression during T-cell activation and proliferation, however, remains unclear, as well as a role for SAMHD1 during HIV-1 pathogenesis. SAMHD1 expression was assessed in CD4 T cells after their activation and in-vitro HIV-1 infection. We performed phenotype analyzes using flow cytometry on CD4 T cells from peripheral blood and lymph nodes from cohorts of HIV-1-infected individuals under antiretroviral treatment or not, and controls. We show that SAMHD1 expression decreased during CD4 T-cell proliferation in association with an increased susceptibility to in-vitro HIV-1 infection. Additionally, circulating memory CD4 T cells are enriched in cells with low levels of SAMHD1. These SAMHD1 cells are highly differentiated, exhibit a large proportion of Ki67 cycling cells and are enriched in T-helper 17 cells. Importantly, memory SAMHD1 cells were depleted from peripheral blood of HIV-infected individuals. We also found that follicular helper T cells present in secondary lymphoid organs lacked the expression of SAMHD1, which was accompanied by a higher susceptibility to HIV-1 infection in vitro. We demonstrate that SAMHD1 expression is decreased during CD4 T-cell activation and proliferation. Also, CD4 T-cell subsets known to be more susceptible to HIV-1 infection, for example, T-helper 17 and follicular helper T cells, display lower levels of SAMHD1. These results pin point a role for SAMHD1 expression in HIV-1 infection and the concomitant depletion of CD4 T cells.
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pasteur-01372413 , version 1 (27-09-2016)

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Nicolas Ruffin, Vedran Brezar, Diana Ayinde, Cécile Lefebvre, Julian Schulze Zur Wiesch, et al.. Low SAMHD1 expression following T-cell activation and proliferation renders CD4+ T cells susceptible to HIV-1.. AIDS. Official journal of the international AIDS Society, 2015, 29 (5), pp.519-30. ⟨10.1097/QAD.0000000000000594⟩. ⟨pasteur-01372413⟩
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