The crystal structure of the catalytic domain of the Ser/Thr kinase PknA from M. tuberculosis shows an Src-like autoinhibited conformation - Institut Pasteur Access content directly
Journal Articles Proteins - Structure, Function and Bioinformatics Year : 2015

The crystal structure of the catalytic domain of the Ser/Thr kinase PknA from M. tuberculosis shows an Src-like autoinhibited conformation

Abstract

Signal transduction mediated by Ser/Thr phosphorylation in Mycobacterium tuberculosis has been intensively studied in the last years, as its genome harbors eleven genes coding for eukaryotic-like Ser/Thr kinases. Here we describe the crystal structure and the autophosphorylation sites of the catalytic domain of PknA, one of two protein kinases essential for pathogen’s survival. The structure of the ligand-free kinase domain shows an auto-inhibited conformation similar to that observed in human Tyr kinases of the Src-family. These results reinforce the high conservation of structural hallmarks and regulation mechanisms between prokaryotic and eukaryotic protein kinases.
Fichier principal
Vignette du fichier
PknA_Prot_SFB_rev_121214.pdf (997.53 Ko) Télécharger le fichier
prot24754-sup-0001-suppinfo1_crystal_structure_wagner.pdf (4.45 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Comment : This is the pre-peer reviewed version of the following article: The crystal structure of the catalytic domain of the ser/thr kinase PknA from M. tuberculosis shows an Src-like autoinhibited conformation, which has been published in final form at http://dx.doi.org/10.1002/prot.24754. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Loading...

Dates and versions

pasteur-01175001 , version 1 (10-07-2015)

Licence

Attribution - NonCommercial - NoDerivatives

Identifiers

Cite

Tristan Wagner, Matthieu Alexandre, Rosario Duran, Nathalie Barilone, Annemarie Wehenkel, et al.. The crystal structure of the catalytic domain of the Ser/Thr kinase PknA from M. tuberculosis shows an Src-like autoinhibited conformation. Proteins - Structure, Function and Bioinformatics, 2015, 83 (5), pp.982-988. ⟨10.1002/prot.24754⟩. ⟨pasteur-01175001⟩
479 View
514 Download

Altmetric

Share

Gmail Facebook X LinkedIn More