Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells. - Institut Pasteur Access content directly
Journal Articles BMC Microbiology Year : 2009

Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells.

Abstract

BACKGROUND: Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epithelial cells. In this study we have analyzed the effect of these drugs on bacterial invasiveness. RESULTS: We demonstrate that INPs affect Chlamydia growth in a dose dependent manner after bacterial invasion. The efficiency of C. trachomatis L2 and C. caviae GPIC entry into host cells was not altered in the presence of INPs. In C. caviae, entry appears to proceed normally with recruitment of actin and the small GTPases Rac, Cdc42 and Arf6 to the site of bacterial entry. CONCLUSION: INPs have a strong inhibitory effect on Chlamydia growth. However, bacterial invasion is not altered in the presence of these drugs. In the light of these results, we discuss several hypotheses regarding the mode of action of INPs on type III secretion during the Chlamydia infectious cycle.
Fichier principal
Vignette du fichier
1471-2180-9-75.pdf (1.44 Mo) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

pasteur-00409865 , version 1 (21-08-2009)

Identifiers

Cite

Sandra Muschiol, Staffan Normark, Birgitta Henriques-Normark, Agathe Subtil. Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells.. BMC Microbiology, 2009, 9, pp.75. ⟨10.1186/1471-2180-9-75⟩. ⟨pasteur-00409865⟩

Collections

PASTEUR CNRS ANR
77 View
191 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More