Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding. - Institut Pasteur Access content directly
Journal Articles Journal of Bacteriology Year : 2007

Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding.

Abstract

On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro.

Dates and versions

pasteur-00366344 , version 1 (06-03-2009)

Identifiers

Cite

Clément Barjon, Karine Wecker, Nadia Izadi-Pruneyre, Philippe Delepelaire. Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding.. Journal of Bacteriology, 2007, 189 (14), pp.5379-82. ⟨10.1128/JB.00251-07⟩. ⟨pasteur-00366344⟩

Collections

PASTEUR CNRS
48 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More