Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone. - Institut Pasteur Access content directly
Journal Articles Journal of Virology Year : 2008

Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone.

Abstract

We and others have uncovered the existence of human T-cell lymphotropic virus type 3 (HTLV-3). We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro, and tax/rex as well as minus-strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24(gag) protein is detected in the cell culture supernatant. Transfection of 293T-long terminal repeat (LTR)-green fluorescent protein (GFP) cells with the HTLV-3 clone promotes formation of syncytia, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.

Dates and versions

pasteur-00363856 , version 1 (24-02-2009)

Identifiers

Cite

Sébastien Alain Chevalier, Nga Ling Ko, Sara Calattini, Adeline Mallet, Marie-Christine Prévost, et al.. Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone.. Journal of Virology, 2008, 82 (13), pp.6747-52. ⟨10.1128/JVI.00247-08⟩. ⟨pasteur-00363856⟩
70 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More