Different GPI-attachment signals affect the oligomerisation of GPI-anchored proteins and their apical sorting. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2008

Different GPI-attachment signals affect the oligomerisation of GPI-anchored proteins and their apical sorting.

Résumé

To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolaterally, respectively, in MDCK cells. We investigated the behaviour of the resulting fusion proteins GFP-FR and GFP-PrP by analysing three parameters: their association with DRMs, their oligomerisation and their apical sorting. Strikingly, we found that different GPI-attachment signals differently modulate the ability of the resulting GFP-fusion protein to oligomerise and to be apically sorted. This is probably owing to differences in the GPI anchor and/or in the surrounding lipid microenvironment. Accordingly, we show that addition of cholesterol to the cells is necessary and sufficient to drive the oligomerisation and consequent apical sorting of GFP-PrP, which under control conditions does not oligomerise and is basolaterally sorted.
Fichier principal
Vignette du fichier
4001.pdf (957.41 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

pasteur-00349337 , version 1 (29-12-2008)

Identifiants

Citer

Simona Paladino, Stephanie Lebreton, Simona Tivodar, Vincenza Campana, Rosaria Tempre, et al.. Different GPI-attachment signals affect the oligomerisation of GPI-anchored proteins and their apical sorting.. Journal of Cell Science, 2008, 121(Pt 24), pp.4001-7. ⟨10.1242/jcs.036038⟩. ⟨pasteur-00349337⟩
111 Consultations
261 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More