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Journal Articles Science Year : 2005

Direct isolation of satellite cells for skeletal muscle regeneration.

Abstract

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

Dates and versions

pasteur-00181349 , version 1 (23-10-2007)

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Didier Montarras, Jennifer Morgan, Charlotte Collins, Frédéric Relaix, Stéphane Zaffran, et al.. Direct isolation of satellite cells for skeletal muscle regeneration.. Science, 2005, 309 (5743), pp.2064-7. ⟨10.1126/science.1114758⟩. ⟨pasteur-00181349⟩

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