Deciphering the role of individual acyl chains in the interaction network between phosphatidylserines and a single-spanning membrane protein. - Institut Pasteur Access content directly
Journal Articles Biochemistry Year : 2002

Deciphering the role of individual acyl chains in the interaction network between phosphatidylserines and a single-spanning membrane protein.

Abstract

PMP1 is a small single-spanning membrane protein functioning as a regulatory subunit of the yeast plasma membrane H(+)-ATPase. This protein forms a unique helix and exhibits a positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines (PSs). A marked groove formed at the helix surface is thought to play a major role in the related lipid-protein interaction network. Mutational analysis and (1)H NMR experiments were therefore performed on a synthetic PMP1 fragment using DPC-d(38) micelles as a membrane-like environment, in the presence of small amounts of POPS. A mutation designed for altering the helix groove was shown to disfavor the POPS binding specificity as much as that affecting the electrostatic interaction network. From POPS titration experiments monitored by a full set of one- and two-dimensional NOESY spectra, the association between the phospholipids and the PMP1 peptide has been followed. Our data reveal that the clustering of POPS molecules is promoted from a stabilized framework obtained by coupling the PMP1 helix groove to a POPS sn-2 chain. To our knowledge, the NOE-based titration plots displayed in this report constitute the first NMR data that directly distinguish the role of the sn-1 and sn-2 acyl chains in a lipid-protein interaction. The results are discussed while taking into account our accurate knowledge of the yeast plasma membrane composition and its ability to form functional lipid rafts.

Dates and versions

pasteur-00166870 , version 1 (10-08-2007)

Identifiers

Cite

Florence Mousson, Yves-Marie Coïc, Françoise Baleux, Veronica Beswick, Alain Sanson, et al.. Deciphering the role of individual acyl chains in the interaction network between phosphatidylserines and a single-spanning membrane protein.. Biochemistry, 2002, 41 (46), pp.13611-6. ⟨10.1021/bi026274b⟩. ⟨pasteur-00166870⟩

Collections

PASTEUR CEA CNRS
83 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More