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Journal Articles Journal of General Virology Year : 2002

Poliovirus transcytosis through M-like cells.


During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer's patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer's patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.



Dates and versions

pasteur-00166731 , version 1 (10-08-2007)



Laurent Ouzilou, Elise Caliot, Isabelle Pelletier, Marie-Christine Prévost, Eric Pringault, et al.. Poliovirus transcytosis through M-like cells.. Journal of General Virology, 2002, 83 (Pt 9), pp.2177-82. ⟨10.1099/0022-1317-83-9-2177⟩. ⟨pasteur-00166731⟩


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