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Journal Articles Journal of General Virology Year : 2002

Poliovirus transcytosis through M-like cells.

Abstract

During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer's patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer's patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.

Domains

Virology

Dates and versions

pasteur-00166731 , version 1 (10-08-2007)

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Cite

Laurent Ouzilou, Elise Caliot, Isabelle Pelletier, Marie-Christine Prévost, Eric Pringault, et al.. Poliovirus transcytosis through M-like cells.. Journal of General Virology, 2002, 83 (Pt 9), pp.2177-82. ⟨10.1099/0022-1317-83-9-2177⟩. ⟨pasteur-00166731⟩

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