How to broaden enzyme substrate specificity: strategies, implications and applications. - Institut Pasteur Access content directly
Journal Articles Research in Microbiology Year : 2005

How to broaden enzyme substrate specificity: strategies, implications and applications.

Jean-Luc Jestin
  • Function : Author
Sophie Vichier-Guerre

Abstract

For identification of mutations associated with the broadening of enzyme substrate specificity, three strategies, including directed enzyme evolution, are described for selected examples. Implications concerning enzyme models are highlighted. Applications to the field of biocatalysis are discussed. A bidimensional map for the classification of enzyme activities is suggested so as to improve genome annotations.

Dates and versions

pasteur-00166275 , version 1 (02-08-2007)

Identifiers

Cite

Jean-Luc Jestin, Sophie Vichier-Guerre. How to broaden enzyme substrate specificity: strategies, implications and applications.. Research in Microbiology, 2005, 156 (10), pp.961-6. ⟨10.1016/j.resmic.2005.09.004⟩. ⟨pasteur-00166275⟩

Collections

PASTEUR CNRS
45 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More