Role of Fyn in the rearrangement of tubulin cytoskeleton induced through TCR. - Institut Pasteur Access content directly
Journal Articles Journal of Immunology Year : 2007

Role of Fyn in the rearrangement of tubulin cytoskeleton induced through TCR.

Abstract

The translocation of the microtubule-organizing center (MTOC), its associated signaling complex, and the secretory apparatus is the most characteristic early event that involves the tubulin cytoskeleton of T or NK cells after their interaction with APC or target cells. Our results show that Fyn kinase activity is essential for MTOC reorientation in an Ag-dependent system. Moreover, T cells from Fyn-deficient mice are unable to rearrange their tubulin cytoskeleton in response to anti-CD3-coated beads. Analysis of conjugates of T cells from transgenic OT-I mice with dendritic cells revealed that an antagonist peptide induces translocation of the MTOC, and that this process is impaired in T cells from Fyn(-/-) OT-I mice. In addition, Fyn deficiency significantly affects the MTOC relocation mediated by agonist peptide stimulation. These results reveal Fyn to be a key regulator of tubulin cytoskeleton reorganization in T cells.

Domains

Immunology

Dates and versions

pasteur-00164640 , version 1 (23-07-2007)

Identifiers

Cite

Noa B Martín-Cófreces, David Sancho, Edgar Fernández, Miguel Vicente-Manzanares, Mónica Gordón-Alonso, et al.. Role of Fyn in the rearrangement of tubulin cytoskeleton induced through TCR.. Journal of Immunology, 2007, 176 (7), pp.4201-7. ⟨10.4049/jimmunol.176.7.4201⟩. ⟨pasteur-00164640⟩

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