Characterization of reemerging chikungunya virus. - Institut Pasteur Access content directly
Journal Articles PLoS Pathogens Year : 2007

Characterization of reemerging chikungunya virus.

Marion Sourisseau
Nicoletta Casartelli
Florence Guivel-Benhassine
Fabien Blanchet
Alessia Zamborlini
Ali Saïb
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Félix A. Rey
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Fernando Arenzana-Seisdedos
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  • PersonId : 853031
Olivier Schwartz
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Abstract

An unprecedented epidemic of chikungunya virus (CHIKV) infection recently started in countries of the Indian Ocean area, causing an acute and painful syndrome with strong fever, asthenia, skin rash, polyarthritis, and lethal cases of encephalitis. The basis for chikungunya disease and the tropism of CHIKV remain unknown. Here, we describe the replication characteristics of recent clinical CHIKV strains. Human epithelial and endothelial cells, primary fibroblasts and, to a lesser extent, monocyte-derived macrophages, were susceptible to infection and allowed viral production. In contrast, CHIKV did not replicate in lymphoid and monocytoid cell lines, primary lymphocytes and monocytes, or monocyte-derived dendritic cells. CHIKV replication was cytopathic and associated with an induction of apoptosis in infected cells. Chloroquine, bafilomycin-A1, and short hairpin RNAs against dynamin-2 inhibited viral production, indicating that viral entry occurs through pH-dependent endocytosis. CHIKV was highly sensitive to the antiviral activity of type I and II interferons. These results provide a general insight into the interaction between CHIKV and its mammalian host.

Domains

Virology
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Dates and versions

pasteur-00161827 , version 1 (30-10-2009)

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Marion Sourisseau, Clémentine Schilte, Nicoletta Casartelli, Céline Trouillet, Florence Guivel-Benhassine, et al.. Characterization of reemerging chikungunya virus.. PLoS Pathogens, 2007, 3 (6), pp.e89. ⟨10.1371/journal.ppat.0030089⟩. ⟨pasteur-00161827⟩
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