Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes. - Institut Pasteur Access content directly
Journal Articles Immunity Year : 2004

Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes.

Abstract

The mechanism by which T cell antigen receptors (TCR) accumulate at the immunological synapse has not been fully elucidated. Since TCRs are continuously internalized and recycled back to the cell surface, we investigated the role of polarized recycling in TCR targeting to the immunological synapse. We show here that the recycling endosomal compartment of T cells encountering activatory antigen-presenting cells (APCs) polarizes towards the T cell-APC contact site. Moreover, TCRs in transit through recycling endosomes are targeted to the immunological synapse. Inhibition of T cell polarity, constitutive TCR endocytosis, or recycling reduces TCR accumulation at the immunological synapse. Conversely, increasing the amount of TCRs in recycling endosomes before synapse formation enhanced their accumulation. Finally, we show that exocytic t-SNAREs from T cells cluster at the APC contact site and that tetanus toxin inhibits TCR accumulation at the immunological synapse, indicating that vesicle fusion mediated by SNARE complexes is involved in TCR targeting to the immunological synapse.

Dates and versions

pasteur-00137478 , version 1 (20-03-2007)

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Vincent Das, Béatrice Nal, Annick Dujeancourt, Maria-Isabel Thoulouze, Thierry Galli, et al.. Activation-induced polarized recycling targets T cell antigen receptors to the immunological synapse; involvement of SNARE complexes.. Immunity, 2004, 20 (5), pp.577-88. ⟨10.1016/S1074-7613(04)00106-2⟩. ⟨pasteur-00137478⟩
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