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Article Dans Une Revue mBio Année : 2024

A CO 2 sensing module modulates β-1,3-glucan exposure in Candida albicans

Qinxi Ma
  • Fonction : Auteur
Emer Hickey
  • Fonction : Auteur
Ian Leaves
  • Fonction : Auteur
Gordon Brown
Neil Gow

Résumé

Microbial species capable of co-existing with healthy individuals, such as the commensal fungus Candida albicans, exploit multifarious strategies to evade our immune defenses. These strategies include the masking of immunoinflammatory pathogen-associated molecular patterns (PAMPs) at their cell surface. We reported previously that C. albicans actively reduces the exposure of the proinflammatory PAMP, β-1,3-glucan, at its cell surface in response to host-related signals such as lactate and hypoxia. Here, we show that clinical isolates of C. albicans display phenotypic variability with respect to their lactate- and hypoxia-induced β-1,3-glucan masking. We have exploited this variability to identify responsive and non-responsive clinical isolates. We then performed RNA sequencing on these isolates to reveal genes whose expression patterns suggested potential association with lactate- or hypoxia-induced β-1,3-glucan masking. The deletion of two such genes attenuated masking: PHO84 and NCE103. We examined NCE103-related signaling further because NCE103 has been shown previously to encode carbonic anhydrase, which promotes adenylyl cyclase-protein kinase A (PKA) signaling at low CO2 levels. We show that while CO2 does not trigger β-1,3-glucan masking in C. albicans, the Sch9-Rca1-Nce103 signaling module strongly influences β-1,3-glucan exposure in response to hypoxia and lactate. In addition to identifying a new regulatory module that controls PAMP exposure in C. albicans, our data imply that this module is important for PKA signaling in response to environmental inputs other than CO2.
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pasteur-04581971 , version 1 (21-05-2024)

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Gabriela Avelar, Arnab Pradhan, Qinxi Ma, Emer Hickey, Ian Leaves, et al.. A CO 2 sensing module modulates β-1,3-glucan exposure in Candida albicans. mBio, 2024, 15 (2), ⟨10.1128/mbio.01898-23⟩. ⟨pasteur-04581971⟩
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