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Article Dans Une Revue Cell Reports Année : 2024

Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation

Résumé

Escape from the bacterial-containing vacuole (BCV) is a key step of Shigella host cell invasion. Rab GTPases subverted to in situ-formed macropinosomes in the vicinity of the BCV have been shown to promote its rupture. The involvement of the BCV itself has remained unclear. We demonstrate that Rab35 is non-canonically entrapped at the BCV. Stimulated emission depletion imaging localizes Rab35 directly on the BCV membranes before vacuolar rupture. The bacterial effector IcsB, a lysine Nε-fatty acylase, is a key regulator of Rab35-BCV recruitment, and we show post-translational acylation of Rab35 by IcsB in its polybasic region. While Rab35 and IcsB are dispensable for the first step of BCV breakage, they are needed for the unwrapping of damaged BCV remnants from Shigella. This provides a framework for understanding Shigella invasion implicating re-localization of a Rab GTPase via its bacteria-dependent post-translational modification to support the mechanical unpeeling of the BCV.
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Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

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pasteur-04533987 , version 1 (05-04-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Nora Mellouk, Arthur Lensen, Noelia Lopez-Montero, Magdalena Gil, Camila Valenzuela, et al.. Post-translational targeting of Rab35 by the effector IcsB of Shigella determines intracellular bacterial niche formation. Cell Reports, 2024, 43 (4), pp.114034. ⟨10.1016/j.celrep.2024.114034⟩. ⟨pasteur-04533987⟩
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