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Article Dans Une Revue Nature Communications Année : 2023

Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants

Volker Thiel
Nicolas Renier

Résumé

Anosmia was identified as a hallmark of COVID-19 early in the pandemic, however, with the emergence of variants of concern, the clinical profile induced by SARS-CoV-2 infection has changed, with anosmia being less frequent. Here, we assessed the clinical, olfactory and neuroinflammatory conditions of golden hamsters infected with the original Wuhan SARS-CoV-2 strain, its isogenic ORF7-deletion mutant and three variants: Gamma, Delta, and Omicron/BA.1. We show that infected animals develop a variant-dependent clinical disease including anosmia, and that the ORF7 of SARS-CoV-2 contributes to the induction of olfactory dysfunction. Conversely, all SARS-CoV-2 variants are neuroinvasive, regardless of the clinical presentation they induce. Taken together, this confirms that neuroinvasion and anosmia are independent phenomena upon SARS-CoV-2 infection. Using newly generated nanoluciferase-expressing SARS-CoV-2, we validate the olfactory pathway as a major entry point into the brain in vivo and demonstrate in vitro that SARS-CoV-2 travels retrogradely and anterogradely along axons in microfluidic neuron-epithelial networks.
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Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

pasteur-04171251 , version 1 (26-07-2023)

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Paternité

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Guilherme Dias de Melo, Victoire Perraud, Flavio Alvarez, Alba Vieites-Prado, Seonhee Kim, et al.. Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants. Nature Communications, 2023, 14 (1), pp.4485. ⟨10.1038/s41467-023-40228-7⟩. ⟨pasteur-04171251⟩
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