HPV genotypes in high-grade cervical lesions and invasive cervical carcinoma detected in Gabonese women - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Infectious Agents and Cancer Année : 2023

HPV genotypes in high-grade cervical lesions and invasive cervical carcinoma detected in Gabonese women

Résumé

Background Cervical cancer is the third most common cancer among women worldwide, but particularly affects women living in sub-Saharan Africa. Screening and vaccination programs are two prevention approaches that can reduce cervical cancer incidence. However, effective vaccination campaigns require better knowledge of the prevalence of the main human papillomavirus (HPV) genotypes reported in high-grade neoplastic lesions and invasive carcinomas in women. Methods All samples collected in this study were processed using standard histopathological methods with haematoxylin and eosin staining of the sections. Areas with abnormal cells were then identified. The HPV genotype was determined on the DNA extracted from the same sections using nested PCR followed by amplicon sequencing and real-time PCR specific to five different HPV genotypes (16, 18, 33, 45 and 58). Results A total of 132 Gabonese patients with high-grade neoplastic lesions were included in this study; 81% were squamous cell carcinomas (SCC). At least one HPV was detected in 92.4% patients; HPV16 (75.4%) was the most frequent genotype, followed by HPV18, 58, 45, 33 and 35. Moreover, histological analysis showed that SCC samples had 50% and 58.2% stage III and IV tumor cells, respectively, according to the FIGO classification. Finally, 36.9% of these stage III and IV patients were less than 50 years old. Conclusions Our results confirm the high prevalence of HPV16 and 18 genotypes among high-grade lesions in Gabonese women. This study confirms the need for a national strategy for early screening of precancerous lesions associated with a broad national vaccination program among non-sexually active women to significantly reduce the long-term cancer burden.
Fichier principal
Vignette du fichier
Moussavou‑Boundzanga2023.pdf (917.23 Ko) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

pasteur-04128009 , version 1 (14-06-2023)

Licence

Paternité

Identifiants

Citer

Pamela Moussavou-Boundzanga, Ismaël Hervé Koumakpayi, Corinne Engohan Aloghe, Junie K Chansi, Rose Revignet, et al.. HPV genotypes in high-grade cervical lesions and invasive cervical carcinoma detected in Gabonese women. Infectious Agents and Cancer, 2023, 18 (1), pp.16. ⟨10.1186/s13027-023-00493-z⟩. ⟨pasteur-04128009⟩
34 Consultations
21 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More