Mitochondrial Dynamics and Activity in Legionella-Infected Cells - Institut Pasteur Access content directly
Book Sections Year : 2019

Mitochondrial Dynamics and Activity in Legionella-Infected Cells

Abstract

The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages.
Fichier principal
Vignette du fichier
2019 Methods Mol Biol_Song.pdf (10.28 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

pasteur-02881368 , version 1 (25-06-2020)

Identifiers

Cite

Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll. Mitochondrial Dynamics and Activity in Legionella-Infected Cells. Carmen Buchrieser; Hubert Hilbi. Legionella: Methods and Protocols, 1921 (13), Springer Science; Business Media, LLC, pp.205-220, 2019, Methods in Molecular Biology (MIMB), 978-1-4939-9047-4 (Print), 978-1-4939-9048-1 (Online). ⟨10.1007/978-1-4939-9048-1_13⟩. ⟨pasteur-02881368⟩
35 View
221 Download

Altmetric

Share

Gmail Facebook X LinkedIn More