SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends - Institut Pasteur Access content directly
Journal Articles Cell Death and Differentiation Year : 2020

SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends

Florence Niedergang

Abstract

Understanding the viral-host cell interface during HIV-1 infection is a prerequisite for the development of innovative antiviral therapies. Here we show that the suppressor of G2 allele of skp1 (SUGT1) is a permissive factor for human immunodeficiency virus (HIV)-1 infection. Expression of SUGT1 increases in infected cells on human brain sections and in permissive host cells. We found that SUGT1 determines the permissiveness to infection of lymphocytes and macrophages by modulating the nuclear import of the viral genome. More importantly, SUGT1 stabilizes the microtubule plus-ends (+MTs) of host cells (through the modulation of microtubule acetylation and the formation of end-binding protein 1 (EB1) comets). This effect on microtubules favors HIV-1 retrograde trafficking and replication. SUGT1 depletion impairs the replication of HIV-1 patient primary isolates and mutant virus that is resistant to raltegravir antiretroviral agent. Altogether our results identify SUGT1 as a cellular factor involved in the post-entry steps of HIV-1 infection that may be targeted for new therapeutic approaches.

Domains

Virology
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Dates and versions

pasteur-02868173 , version 1 (18-06-2020)

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Awatef Allouch, Cristina Di Primio, Audrey Paoletti, Gabrielle Lê-Bury, Frédéric Subra, et al.. SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends. Cell Death and Differentiation, 2020, Online ahead of print, pp.18 - 22. ⟨10.1038/s41418-020-0573-5⟩. ⟨pasteur-02868173⟩
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