Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin - Institut Pasteur Access content directly
Journal Articles Nature Communications Year : 2018

Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin

Abstract

Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus type 5 (Ad5) virus to show that they adopt different strategies in their respective positioning at active chromatin. HBV contacts preferentially CpG islands (CGIs) enriched in Cfp1 a factor required for its transcription. These CGIs are often associated with highly expressed genes (HEG) and genes deregulated during infection. Ad5 DNA interacts preferentially with transcription start sites (TSSs) and enhancers of HEG, as well as genes upregulated during infection. These results show that DNA viruses use different strategies to infiltrate genomic 3D networks and target specific regions. This targeting may facilitate the recruitment of transcription factors necessary for their own replication and contribute to the deregulation of cellular gene expression.

Domains

Virology
Fichier principal
Vignette du fichier
Tridimensional infiltration of DNA viruses into the host genome.pdf (6.34 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-02866759 , version 1 (12-06-2020)

Licence

Attribution

Identifiers

Cite

Pierrick Moreau, Axel Cournac, Gianna Aurora Palumbo, Martial Marbouty, Shogofa Mortaza, et al.. Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin. Nature Communications, 2018, 9 (1), pp.4268. ⟨10.1038/s41467-018-06739-4⟩. ⟨pasteur-02866759⟩
50 View
71 Download

Altmetric

Share

Gmail Facebook X LinkedIn More