Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles.
Résumé
Recent advances in nanotechnology have seen the development of a number of microbiocidal and/or anti-adhesive nanoparticles displaying activity against biofilms. In this work, trimeric thiomannoside clusters conjugated to nanodiamond particles (ND) were targeted for investigation. NDs have attracted attention as a biocompatible nanomaterial and we were curious to see whether the high mannose glycotope density obtained upon grouping monosaccharide units in triads might lead to the corresponding ND-conjugates behaving as effective inhibitors of E. coli type 1 fimbriae-mediated adhesion as well as of biofilm formation. The required trimeric thiosugar clusters were obtained through a convenient thiol-ene "click" strategy and were subsequently conjugated to alkynyl-functionalized NDs using a Cu(I)-catalysed "click" reaction. We demonstrated that the tri-thiomannoside cluster-conjugated NDs (ND-Man3) show potent inhibition of type 1 fimbriae-mediated E. coli adhesion to yeast and T24 bladder cells as well as of biofilm formation. The biofilm disrupting effects demonstrated here have only rarely been reported in the past for analogues featuring such simple glycosidic motifs. Moreover, the finding that the tri-thiomannoside cluster (Man3N3) is itself a relatively efficient inhibitor, even when not conjugated to any ND edifice, suggests that alternative mono- or multivalent sugar-derived analogues might also be usefully explored for E. coli-mediated biofilm disrupting properties.
Mots clés
Glycosides/chemistry
Magnetic Resonance Spectroscopy
Mannosides/*chemistry
Solvents/chemistry
Nanoparticles/*chemistry
Nanotechnology
Particle Size
Photoelectron Spectroscopy
Nanoconjugates
Bacterial/*metabolism
Fimbriae
Escherichia coli/*metabolism
Diamond/*chemistry
Dimerization
Thin Layer
Chromatography
Tumor
Cell Line
Carbohydrates/chemistry
Biocompatible Materials/chemistry
Bacterial Adhesion
*Biofilms
Humans
Green Fluorescent Proteins/metabolism
Domaines
BactériologieOrigine | Fichiers produits par l'(les) auteur(s) |
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