Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile - Institut Pasteur Access content directly
Journal Articles mBio Year : 2015

Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile

Abstract

Clostridium difficile is the cause of most frequently occurring nosocomial diarrhea worldwide. As an enteropatho-gen, C. difficile must be exposed to multiple exogenous genetic elements in bacteriophage-rich gut communities. CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems allow bacteria to adapt to foreign genetic invaders. Our recent data revealed active expression and processing of CRISPR RNAs from multiple type I-B CRISPR arrays in C. difficile reference strain 630. Here, we demonstrate active expression of CRISPR arrays in strain R20291, an epidemic C. difficile strain. Through genome sequencing and host range analysis of several new C. difficile phages and plasmid conjugation experiments, we provide evidence of defensive function of the CRISPR-Cas system in both C. difficile strains. We further demonstrate that C. difficile Cas proteins are capable of interference in a heterologous host, Escherichia coli. These data set the stage for mechanistic and physiological analyses of CRISPR-Cas-mediated interactions of important global human pathogen with its genetic parasites. IMPORTANCE Clostridium difficile is the major cause of nosocomial infections associated with antibiotic therapy worldwide. To survive in bacteriophage-rich gut communities, enteropathogens must develop efficient systems for defense against foreign DNA elements. CRISPR-Cas systems have recently taken center stage among various anti-invader bacterial defense systems. We provide experimental evidence for the function of the C. difficile CRISPR system against plasmid DNA and bacteriophages. These data demonstrate the original features of active C. difficile CRISPR system and bring important insights into the interactions of this major enteropathogen with foreign DNA invaders during its infection cycle.
Fichier principal
Vignette du fichier
mBio-2015-Boudry.pdf (3.72 Mo) Télécharger le fichier
mbo004152441sf1.pdf (583.32 Ko) Télécharger le fichier
mbo004152441sf2.pdf (321.43 Ko) Télécharger le fichier
mbo004152441sf3.pdf (62.06 Ko) Télécharger le fichier
mbo004152441st1.pdf (91.41 Ko) Télécharger le fichier
mbo004152441st2.pdf (302.75 Ko) Télécharger le fichier
mbo004152441st3.pdf (99.47 Ko) Télécharger le fichier
mbo004152441st4.pdf (135.16 Ko) Télécharger le fichier
mbo004152441st5.pdf (123.85 Ko) Télécharger le fichier
mbo004152441st6.pdf (69.79 Ko) Télécharger le fichier
Origin Publication funded by an institution
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Publisher files allowed on an open archive
Origin Files produced by the author(s)

Dates and versions

pasteur-01370739 , version 1 (23-09-2016)

Licence

Identifiers

Cite

Pierre Boudry, Ekaterina Semenova, Marc Monot, Kirill A. Datsenko, Anna Lopatina, et al.. Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile. mBio, 2015, 6 (5), pp.e01112-15. ⟨10.1128/mBio.01112-15⟩. ⟨pasteur-01370739⟩
170 View
646 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More