Hazard assessment of hexagonal boron nitride and hexagonal boron nitride reinforced thermoplastic polyurethane composites using human skin and lung cells - Archive ouverte du site Alsace Accéder directement au contenu
Article Dans Une Revue Journal of Hazardous Materials Année : 2024

Hazard assessment of hexagonal boron nitride and hexagonal boron nitride reinforced thermoplastic polyurethane composites using human skin and lung cells

Antonio Esau Del Rio Castillo
  • Fonction : Auteur
Sanjay B Thorat
  • Fonction : Auteur
Francesco Bonaccorso
  • Fonction : Auteur
Andrea Armirotti
  • Fonction : Auteur
Bengt Fadeel
  • Fonction : Auteur
  • PersonId : 922751

Résumé

Hexagonal boron nitride (hBN) is an emerging two-dimensional material attracting considerable attention in the industrial sector given its innovative physicochemical properties. Potential risks are associated mainly with occupational exposure where inhalation and skin contact are the most relevant exposure routes for workers. Here we aimed at characterizing the effects induced by composites of thermoplastic polyurethane (TPU) and hBN, using immortalized HaCaT skin keratinocytes and BEAS-2B bronchial epithelial cells. The composite was abraded using a Taber® rotary abraser and abraded TPU and TPU-hBN were also subjected to photo-Fenton-mediated degradation mimicking potential weathering across the product life cycle. Cells were exposed to the materials for 24 h (acute exposure) or twice per week for 4 weeks (chronic exposure) and evaluated with respect to material internalization, cytotoxicity, and proinflammatory cytokine secretion. Additionally, comprehensive mass spectrometry-based proteomics and metabolomics (secretomics) analyses were performed. Overall, despite evidence of cellular uptake of the material, no significant cellular and/or protein expression profiles alterations were observed after acute or chronic exposure of HaCaT or BEAS-2B cells, identifying only few pro-inflammatory proteins. Similar results were obtained for the degraded materials. These results support the determination of hazard profiles associated with cutaneous and pulmonary hBN-reinforced polymer composites exposure.
Fichier principal
Vignette du fichier
Carlin et al. J. Hazard. Mater. 2024 N346.pdf (12.58 Mo) Télécharger le fichier
Origine Publication financée par une institution

Dates et versions

hal-04659634 , version 1 (23-07-2024)

Identifiants

Citer

Michela Carlin, Jasreen Kaur, Dinu Zinovie Ciobanu, Zhengmei Song, Magnus Olsson, et al.. Hazard assessment of hexagonal boron nitride and hexagonal boron nitride reinforced thermoplastic polyurethane composites using human skin and lung cells. Journal of Hazardous Materials, 2024, 473, pp.134686. ⟨10.1016/j.jhazmat.2024.134686⟩. ⟨hal-04659634⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More