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            <funder>This study was supported by Agence nationale de la recherche (ANR) (MolecularXiv” Grant number: ANR-22-PEXM-0002, and the program ‘‘Investissements d’Avenir’’ launched by the French Government and implemented by the ANR with the reference: ANR-10-IDEX-0001−02 PSL), Joe W. and Dorothy Dorsett Brown Foundation (exploratory and advanced grants), Université Paris-Est Créteil Val-de-Marne (PhD fellowship), European Research Council (Consolidator Grant Photo-MedMet to G.G. (GA 681679)),</funder>
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                <term xml:lang="en">modified oligonucleotides</term>
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                <term xml:lang="en">photodynamic therapy (PDT)</term>
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              <p>Alternative methods for the preparation of naïve libraries for SELEX experiments are in dire need, particularly when hydrophobic, bulky, and complex modification patterns are considered. Here, we explore first steps towards the preparation of libraries equipped with ruthenium polypyridyl complexes using Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC) reactions. We demonstrated that dsDNA products can be efficiently equipped with dibenzocyclooctyne (DIBAC) moieties using a suitable modified nucleoside triphosphate and PEX reactions or PCR. The resulting dsDNA products can then be further modified using SPAAC and ruthenium polypyridyl complexes equipped with azide moieties, permitting the installation of &amp;gt;10 complexes. Finally, dsDNA can be efficiently converted into the corresponding modified ssDNA using magnetoseparation. These results offer the possibility of producing longer oligonucleotides equipped with complex modification patterns and open the way to SPAACclick-SELEX methodologies</p>
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