Size-dependent temporal decoupling of morphogenesis and transcriptional programs in gastruloids - Institut Pasteur
Pré-Publication, Document De Travail Année : 2024

Size-dependent temporal decoupling of morphogenesis and transcriptional programs in gastruloids

Résumé

Understanding the interplay between cell fate specification and morphogenetic changes remains a central challenge in developmental biology. Gastruloids, self-organizing stem cell-based models of post-implantation mammalian development, provide a powerful platform to address this question. Here, we show that physical parameters, particularly system size, critically influence the timing and outcomes of morphogenetic processes. Larger gastruloids exhibit delayed symmetry breaking, increased multipolarity, and prolonged axial elongation, with morphogenesis driven by system size. Despite these variations, transcriptional programs and cell fate composition remain remarkably stable across a broad size range. Notably, extreme sizes show distinct transcriptional modules and clear shifts in gene expression patterns. Intriguingly, size perturbation experiments rescued the morphogenetic and pattern phenotypes observed in extreme sizes, demonstrating the remarkable adaptability of gastruloids to their effective system size. These findings establish gastruloids as versatile models for studying spatiotemporal dynamics in mammalian embryogenesis and reveal how physical constraints decouple transcriptional from morphogenetic programs.
Fichier principal
Vignette du fichier
2024.12.23.630037v1.full.pdf (22.94 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

pasteur-04876299 , version 1 (09-01-2025)

Licence

Identifiants

Citer

Isma Bennabi, Pauline Hansen, Melody Merle, Judith Pineau, Lucille Lopez-Delisle, et al.. Size-dependent temporal decoupling of morphogenesis and transcriptional programs in gastruloids. 2024. ⟨pasteur-04876299⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More