%0 Journal Article %T Saliva for molecular detection of SARS‐CoV ‐2 in pre‐school and school‐age children %+ Département Plateforme (PF I2BC) %+ CHU Necker - Enfants Malades [AP-HP] %+ CIC - Mère Enfant Necker Cochin Paris Centre (CIC 1419) %+ Immunogenetics of pediatric autoimmune diseases (Equipe Inserm U1163) %+ AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre) %+ Hôpital Louis Mourier - AP-HP [Colombes] %+ Hôpital Ambroise Paré [AP-HP] %+ AP-HP - Hôpital Antoine Béclère [Clamart] %+ CHU Trousseau [APHP] %+ Ministère de l'Éducation nationale %+ Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur) %+ Biologie des ARN et virus influenza - RNA Biology of Influenza Virus (CNRS-UMR3569) %+ Hôpital Jean Verdier [AP-HP] %+ Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française] %+ Unité de recherche clinique / Centre d'investigation clinique [CHU Necker] %+ Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur) %+ Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)) %+ Université Paris Cité (UPCité) %+ Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)) %A Delaunay-Moisan, Agnes %A Guilleminot, Tiffany %A Semeraro, Michaela %A Briand, Nelly %A Bader-Meunier, Brigitte %A Berthaud, Romain %A Morelle, Guillaume %A Quartier, Pierre %A Galeotti, Caroline %A Basmaci, Romain %A Benoist, Gregoire %A Gajdos, Vincent %A Lorrot, Mathie %A Rifai, Mahmoud %A Crespin, Matis %A M'Sakni, Zakary %A Padavia, Faheemah %A Savetier-Leroy, Catherine %A Lorenzi, Michelle %A Maurin, Caroline %A Behillil, Sylvie %A de Pontual, Loic %A Elenga, Narcisse %A Bouazza, Naim %A Moltrecht, Brigitte %A van der Werf, Sylvie %A Leruez-Ville, Marianne %A Sermet-Gaudelus, Isabelle %Z This study was funded by a research grant from the French Ministry of Health (PHRC) and sponsored by the Assistance Publique – Hôpitaux de Paris (AP-HP) and by delegation: Délégation à la Recherche Clinique et à l'Innovation - DRCI (Clinical Research and Innovation Department). %< avec comité de lecture %@ 1462-2912 %J Environmental Microbiology %I Society for Applied Microbiology and Wiley-Blackwell %S Thematic Issue on Pathogen and Antimicrobial Resistance Ecology %V 24 %N 10 %P 4725-4737 %8 2022-10 %D 2022 %R 10.1111/1462-2920.16151 %M 36065993 %Z Life Sciences [q-bio]Journal articles %X SARS-CoV-2 diagnosis is a cornerstone for the management of coronavirus disease 2019 (COVID-19). Numerous studies have assessed saliva performance over nasopharyngeal sampling (NPS), but data in young children are still rare. We explored saliva performance for SARS-CoV-2 detection by RT-PCR according to the time interval from initial symptoms or patient serological status. We collected 509 NPS and saliva paired samples at initial diagnosis from 166 children under 12 years of age (including 57 children under 6), 106 between 12 and 17, and 237 adults. In children under 12, overall detection rate for SARS-CoV-2 was comparable in saliva and NPS, with an overall agreement of 89.8%. Saliva sensitivity was significantly lower than that of NPS (77.1% compared to 95.8%) in pre-school and school-age children but regained 96% when considering seronegative children only. This pattern was also observed to a lesser degree in adolescents but not in adults. Sensitivity of saliva was independent of symptoms, in contrary to NPS, whose sensitivity decreased significantly in asymptomatic subjects. Performance of saliva is excellent in children under 12 at early stages of infection. This reinforces saliva as a collection method for early and unbiased SARS-CoV-2 detection and a less invasive alternative for young children. %G English %Z The authors thank all the families for their participation and URC-CIC Paris Centre for the implementation, monitoring and data management of the study. The later includes Jean-Marc Treluyer and Caroline Elie, Mathilde Serpinet, Céline Hauw, Amel Bouheraoua, Victor Bruyère, Mégane Ribot, Rosa Salhi, Gael Plastow, Cindy Parent, Sonia Lounis, Insaf Berrazaga, Wafa Atmani, Amélie de Haut de Sigy, Geovanna Meneses and Fabrice Gourmelon. The authors thank Carl Mann for careful reading of the manuscript. %2 https://pasteur.hal.science/pasteur-04097919/document %2 https://pasteur.hal.science/pasteur-04097919/file/Environmental%20Microbiology%20-%202022%20-%20Delaunay%E2%80%90Moisan%20-%20Saliva%20for%20molecular%20detection%20of%20SARS%E2%80%90CoV%E2%80%902%20in%20pre%E2%80%90school%20and.pdf %L pasteur-04097919 %U https://pasteur.hal.science/pasteur-04097919 %~ PASTEUR %~ CEA %~ CNRS %~ APHP %~ CEA-UPSAY %~ I2BC %~ UNIV-PARIS-SACLAY %~ JOLIOT %~ CEA-DRF %~ CHU-UNIV-PARIS5 %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-MEDECINE %~ SU-MED %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ UP-SANTE %~ UP-SCIENCES %~ UNIVERSITE-PARIS-SACLAY %~ SU-TI %~ GS-BIOSPHERA %~ GS-LIFE-SCIENCES-HEALTH %~ GS-HEALTH-DRUG-SCIENCES %~ ALLIANCE-SU %~ RNA-BIO-INF-VIR %~ PASTEUR_UMR3569 %~ GMV-ARN