%0 Journal Article %T Bacteriophages benefit from generalized transduction %+ University of Glasgow %+ University of Copenhagen = Københavns Universitet (UCPH) %+ Génomique évolutive des Microbes / Microbial Evolutionary Genomics %+ CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology [Shanghai] %+ University of Oxford %A Fillol-Salom, Alfred %A Alsaadi, Ahlam %A Moura de Sousa, Jorge, A %A Zhong, Li %A Foster, Kevin, R %A Rocha, Eduardo, P C %A Penadés, José, R %A Ingmer, Hanne %A Haaber, Jakob %Z This work was funded by a DFF Sapere Aude Young Elite Reasearcher grant to JH, by the Danish National Research Foundation (DNRF120) to HI, from an EU FP7 PRESTIGE grant [PRESTIGE-2017-1-0012] from Campus France to JAMS, and grants from the Medical Research Council (MRC, UK) [MR/S00940X/1], from the Biotechnology and Biological Sciences Research Council (BBSRC, UK) [BB/S003835/1], from the European Union [ERC-ADG-2014 Proposal n◦ 670932 Dut-signal], and from Wellcome Trust (201531/Z/16Z) to JRP. An ANR grant Salmo_Prophages [ANR-16-CE12-29] to EPCR. KRF is funded by European Research Council Grant 787932 and Wellcome Trust Investigator award 209397/Z/17/Z. %< avec comité de lecture %@ 1553-7366 %J PLoS Pathogens %I Public Library of Science %V 15 %N 7 %P e1007888 %8 2019-07-05 %D 2019 %R 10.1371/journal.ppat.1007888 %M 31276485 %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/Genetics %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Temperate phages are bacterial viruses that as part of their life cycle reside in the bacterial genome as prophages. They are found in many species including most clinical strains of the human pathogens, Staphylococcus aureus and Salmonella enterica serovar Typhimurium. Previously, temperate phages were considered as only bacterial predators, but mounting evidence point to both antagonistic and mutualistic interactions with for example some temperate phages contributing to virulence by encoding virulence factors. Here we show that generalized transduction, one type of bacterial DNA transfer by phages, can create conditions where not only the recipient host but also the transducing phage benefit. With antibiotic resistance as a model trait we used individual-based models and experimental approaches to show that antibiotic susceptible cells become resistant to both antibiotics and phage by i) integrating the generalized transducing temperate phages and ii) acquiring transducing phage particles carrying antibiotic resistance genes obtained from resistant cells in the environment. This is not observed for non-generalized transducing temperate phages, which are unable to package bacterial DNA, nor for generalized transducing virulent phages that do not form lysogens. Once established, the lysogenic host and the prophage benefit from the existence of transducing particles that can shuffle bacterial genes between lysogens and for example disseminate resistance to antibiotics, a trait not encoded by the phage. This facilitates bacterial survival and leads to phage population growth. We propose that generalized transduction can function as a mutualistic trait where temperate phages cooperate with their hosts to survive in rapidly-changing environments. This implies that generalized transduction is not just an error in DNA packaging but is selected for by phages to ensure their survival. %G English %2 https://pasteur.hal.science/pasteur-04076056/document %2 https://pasteur.hal.science/pasteur-04076056/file/Fillol-Salom.PlosP.19.transduction.pdf %L pasteur-04076056 %U https://pasteur.hal.science/pasteur-04076056 %~ PASTEUR %~ CNRS %~ OPENAIRE %~ ANR %~ MICROBIAL-EVOLUTIONARY-GENOMICS %~ UMR3525