%0 Unpublished work %T Diachronic monitoring of snow leopards at Sarychat-Ertash State Reserve (Kyrgyzstan) through scat genotyping: a pilot study %+ Objectif Sciences International NGO (OSI-NGO) %+ WildMap Consulting Ltd %+ Génétique humaine et fonctions cognitives - Human Genetics and Cognitive Functions (GHFC (UMR_3571 / U-Pasteur_1)) %+ Natural Resources DNA Profiling and Forensic Centre (NRDPFC) %+ Sarychat-Ertash State Reserve %+ Université Paris Diderot - Paris 7 (UPD7) %+ Evolution, génomes, comportement et écologie (EGCE) %A Rode, Julie %A Pelletier, Agnès %A Fumey, Julien %A Rode, Stéphane %A Cabanat, Anne-Lise %A Ouvrard, Anne %A Chaix, Bastien %A White, Bradley %A Harnden, Matthew %A Xuan, Nguyen, Thi %A Vereshagin, Alexander %A Casane, Didier %Z This study was supported by the Natural Resource DNA and Profiling Forensic Center (19,919.33 $CA), the Endangered Species Reserve Fund Program of the Toronto Zoo (1,000 $CA), the PPG Conservation and Sustainability Fund of the Pittsburgh Zoo & PPG Aquarium (1,500 $US), and by a Faculty Research grant from the Canadian Mennonite University (2,500 $CA). This study was funded thanks to the citizens which took part in the many expeditions. %Z Posted April 30, 2020 on bioRxiv. %8 2020-04-30 %D 2020 %R 10.1101/835025 %K noninvasive genotyping %K population dynamics %K microsatellite %K relatedness %K diachronic monitoring %K citizen science %K Central Asia %K snow leopard %Z Life Sciences [q-bio]/Animal biology/Vertebrate ZoologyPreprints, Working Papers, ... %X Snow leopards ( Panthera uncia ) are a keystone species of Central Asia’s high mountain ecosystem. The species is listed as vulnerable and is elusive, preventing accurate population assessments that could inform conservation actions. Non-invasive genetic monitoring conducted by citizen scientists offers avenues to provide key data on this species that would otherwise be inaccessible. From 2011 to 2015, OSI-Panthera citizen science expeditions tracked signs of presence of snow leopards along transects in the main valleys and crests of the Sarychat-Ertash State Reserve (Kyrgyzstan). Scat samples were genotyped at seven autosomal microsatellite loci and at a X/Y locus for sex identification, which allowed estimating a minimum of 11 individuals present in the reserve from 2011 to 2015. The genetic recapture of 7 of these individuals enabled diachronic monitoring, providing indications of individuals’ movements throughout the reserve. We found putative family relationships between several individuals. Our results demonstrate the potential of this citizen science program to get a precise description of a snow leopard population through time. %G English %2 https://pasteur.hal.science/pasteur-04067170/document %2 https://pasteur.hal.science/pasteur-04067170/file/835025v2.full.pdf %L pasteur-04067170 %U https://pasteur.hal.science/pasteur-04067170 %~ IRD %~ PASTEUR %~ UNIV-PARIS7 %~ CNRS %~ USPC %~ UNIV-PARIS %~ UP-SCIENCES %~ EGCE %~ GS-BIOSPHERA %~ TEST3-HALCNRS %~ TEST4-HALCNRS %~ HUM-GEN-COGN-FUN %~ PASTEUR_UMR3571 %~ TEST5-HALCNRS