%0 Journal Article %T Genetics and immunity of Anopheles response to the entomopathogenic fungus Metarhizium anisopliae overlap with immunity to Plasmodium %+ International Centre of Insect Physiology and Ecology (ICIPE) %+ Mycologie moléculaire - Molecular Mycology %+ Génétique et Génomique des Insectes Vecteurs - Genetics and Genomics of Insect Vectors %+ Medical College of Wisconsin [Milwaukee] (MCW) %A Bukhari, Tullu %A Aimanianda, Vishukumar %A Bischoff, Emmanuel %A Brito-Fravallo, Emma %A Eiglmeier, Karin %A Riehle, Michelle %A Vernick, Kenneth %A Mitri, Christian %Z This study received financial support to KV from the European Commission Horizon 2020 Infrastructures #731060 Infravec2; European Research Council Support for Frontier Research, Advanced Grant #323173 AnoPath; Agence Nationale de la Recherche #ANR-19-CE35-0004 ArboVec; to MMR from National Institutes of Health, NIAID #AI145999; and French Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” #ANR-10-LABX-62-IBEID. %< avec comité de lecture %@ 2045-2322 %J Scientific Reports %I Nature Publishing Group %V 12 %N 1 %P 6315 %8 2022-12 %D 2022 %R 10.1038/s41598-022-10190-3 %M 35428783 %Z Life Sciences [q-bio]Journal articles %X Entomopathogenic fungi have been explored as a potential biopesticide to counteract the insecticide resistance issue in mosquitoes. However, little is known about the possibility that genetic resistance to fungal biopesticides could evolve in mosquito populations. Here, we detected an important genetic component underlying Anopheles coluzzii survival after exposure to the entomopathogenic fungus Metarhizium anisopliae . A familiality study detected variation for survival among wild mosquito isofemale pedigrees, and genetic mapping identified two loci that significantly influence mosquito survival after fungus exposure. One locus overlaps with a previously reported locus for Anopheles susceptibility to the human malaria parasite Plasmodium falciparum . Candidate gene studies revealed that two LRR proteins encoded by APL1C and LRIM1 genes in this newly mapped locus are required for protection of female A. coluzzii from M. anisopliae , as is the complement-like factor Tep1. These results indicate that natural Anopheles populations already segregate frequent genetic variation for differential mosquito survival after fungal challenge and suggest a similarity in Anopheles protective responses against fungus and Plasmodium . However, this immune similarity raises the possibility that fungus-resistant mosquitoes could also display enhanced resistance to Plasmodium , suggesting an advantage of selecting for fungus resistance in vector populations to promote naturally diminished malaria vector competence. %G English %Z We thank Dr. N.K. Maniania for the provision of spores of M. anisopliae (ICIPE30). %2 https://pasteur.hal.science/pasteur-03651757/document %2 https://pasteur.hal.science/pasteur-03651757/file/s41598-022-10190-3.pdf %L pasteur-03651757 %U https://pasteur.hal.science/pasteur-03651757 %~ PASTEUR %~ CNRS %~ OPENAIRE %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ ANR %~ TEST3-HALCNRS %~ TEST4-HALCNRS %~ MYCO-MOL %~ GEN-GENO-INSECT-VECT %~ PASTEUR_UMR2000 %~ MYCO-MOL-CNRMA %~ TEST5-HALCNRS