%0 Journal Article %T De novo synthesized polyunsaturated fatty acids operate as both host immunomodulators and nutrients for Mycobacterium tuberculosis %+ Immunobiologie de l'Infection - Immunobiology of Infection %+ Université Paris Cité (UPCité) %+ Université Sorbonne Paris Cité (USPC) %+ Institut de pharmacologie et de biologie structurale (IPBS) %+ Pathogénomique mycobactérienne intégrée - Integrated Mycobacterial Pathogenomics %+ Immunorégulation - Immunoregulation %+ MetaboHUB-MetaToul %+ Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC) %A Laval, Thomas %A Pedró-Cos, Laura %A Malaga, Wladimir %A Guenin-Macé, Laure %A Pawlik, Alexandre %A Mayau, Véronique %A Yahia-Cherbal, Hanane %A Delos, Océane %A Frigui, Wafa %A Bertrand-Michel, Justine %A Guilhot, Christophe %A Demangel, Caroline %Z This study was supported by the Institut Pasteur (TL, CD) and INSERM (U1224, CD). %< avec comité de lecture %J eLife %I eLife Sciences Publication %V 10 %P e71946 %8 2021-12-24 %D 2021 %R 10.7554/eLife.71946 %M 34951591 %K Mycobacterium tuberculosis %K fatty acids %K immunology %K immunomodulation %K infectious disease %K inflammation %K innate immunity %K macrophages %K microbiology %K mouse %K nutrient. %K nutrient %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/ImmunologyJournal articles %X Successful control of Mycobacterium tuberculosis (Mtb) infection by macrophages relies on immunometabolic reprogramming, where the role of fatty acids (FAs) remains poorly understood. Recent studies unraveled Mtb’s capacity to acquire saturated and monounsaturated FAs via the Mce1 importer. However, upon activation, macrophages produce polyunsaturated fatty acids (PUFAs), mammal-specific FAs mediating the generation of immunomodulatory eicosanoids. Here, we asked how Mtb modulates de novo synthesis of PUFAs in primary mouse macrophages and whether this benefits host or pathogen. Quantitative lipidomics revealed that Mtb infection selectively activates the biosynthesis of ω6 PUFAs upstream of the eicosanoid precursor arachidonic acid (AA) via transcriptional activation of Fads2 . Inhibiting FADS2 in infected macrophages impaired their inflammatory and antimicrobial responses but had no effect on Mtb growth in host cells nor mice. Using a click-chemistry approach, we found that Mtb efficiently imports ω6 PUFAs via Mce1 in axenic culture, including AA. Further, Mtb preferentially internalized AA over all other FAs within infected macrophages by mechanisms partially depending on Mce1 and supporting intracellular persistence. Notably, IFNγ repressed de novo synthesis of AA by infected mouse macrophages and restricted AA import by intracellular Mtb. Together, these findings identify AA as a major FA substrate for intracellular Mtb, whose mobilization by innate immune responses is opportunistically hijacked by the pathogen and downregulated by IFNγ. %G English %Z We thank the Cytometry and Biomarkers (UTechS CB) Technological Platform for help with nCounter FLEX NanoString use and the Image Analysis Hub of Institut Pasteur for confocal microscopy data analysis. We gratefully acknowledge the MetaToul (Toulouse metabolomics and fluxomics facilities, https://www.metatoul.fr), part of the French National Infrastructure for Metabolomics and Fluxomics MetaboHUB-ANR-11-INBS-0010, for lipid analysis. We also thank Prof. Luke Chamberlain (University of Strathclyde, Glasgow, UK) for sharing his expertise of FA labeling by click-chemistry. %2 https://pasteur.hal.science/pasteur-03534651/document %2 https://pasteur.hal.science/pasteur-03534651/file/elife-71946-v2.pdf %L pasteur-03534651 %U https://pasteur.hal.science/pasteur-03534651 %~ INSERM %~ PASTEUR %~ UNIV-TLSE3 %~ CNRS %~ APHP %~ AGREENIUM %~ INRAE %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ IPBS %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ TEST3-HALCNRS %~ METABOHUB %~ I2MC %~ FRM %~ ENVT