%0 Journal Article %T Whole genome sequencing reveals resemblance between ESBL-producing and carbapenem resistant Klebsiella pneumoniae isolates from Austrian rivers and clinical isolates from hospitals %+ Austrian Agency for Health and Food Safety (AGES) %+ Vienna University of Technology (TU Wien) %+ Ordensklinikum Linz Elisabethinen %+ Klinikum Klagenfurt am Wörthersee %+ Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens %+ Universität für Bodenkultur Wien = University of Natural Resources and Life [Vienne, Autriche] (BOKU) %A Lepuschitz, Sarah %A Schill, Simone %A Stoeger, Anna %A Pekard-Amenitsch, Shiva %A Huhulescu, Steliana %A Inreiter, Norbert %A Hartl, Rainer %A Kerschner, Heidrun %A Sorschag, Sieglinde %A Springer, Burkhard %A Brisse, Sylvain %A Allerberger, Franz %A Mach, Robert, L. %A Ruppitsch, Werner %Z This work was partially funded by a grant to S.L. by the Austrian Society for Antimicrobial Chemotherapy (ÖGACH). Part of the sequencing-work was supported financially by the MedVetKlebs project, a component of the One Health European Joint Programme, which has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No 773830. %< avec comité de lecture %@ 0048-9697 %J Science of the Total Environment %I Elsevier %V 662 %P 227-235 %8 2019-01-20 %D 2019 %R 10.1016/j.scitotenv.2019.01.179 %M 30690357 %K Anthropogenic pollution %K Multiresistance %K Surface-water %K Surveillance %K Whole-genome sequencing %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology %Z Life Sciences [q-bio]/Biodiversity %Z Life Sciences [q-bio]/Santé publique et épidémiologieJournal articles %X In 2016, the Austrian Agency for Health and Food Safety started a pilot project to investigate antimicrobial resistance in surface water. Here we report on the characterisation of carbapenem resistant and ESBL-producing K. pneumoniae isolates from Austrian river water samples compared to 95 clinical isolates recently obtained in Austrian hospitals. Ten water samples were taken from four main rivers, collected upstream and downstream of major cities in 2016. For subtyping and comparison, public core genome multi locus sequence typing (cgMLST) schemes were used. The presence of AMR genes, virulence genes and plasmids was extracted from whole genome sequence (WGS) data. In total three ESBL-producing strains and two carbapenem resistant strains were isolated. WGS based comparison of these five water isolates to 95 clinical isolates identified three clusters. Cluster 1 (ST11) and cluster 2 (ST985) consisted of doublets of carbapenem resistant strains (one water and one clinical isolate each). Cluster 3 (ST405) consisted of three ESBL-producing strains isolated from one water sample and two clinical specimens. The cities, in which patient isolates of cluster 2 and 3 were collected, were in concordance with the water sampling locations downstream from these cities. The genetic concordance between isolates from river water samples and patient isolates raises concerns regarding the release of wastewater treatment plant effluents into surface water. From a public health perspective these findings demand attention and strategies are required to minimize the spread of multiresistant strains to the environment. %G English %Z We thank the team of curators of the Institut Pasteur MLST and whole genome MLST databases for curating the data and making them publicly available at http://bigsdb.pasteur.fr/. %2 https://pasteur.hal.science/pasteur-03329691/document %2 https://pasteur.hal.science/pasteur-03329691/file/1-s2.0-S0048969719301986-main.pdf %L pasteur-03329691 %U https://pasteur.hal.science/pasteur-03329691 %~ PASTEUR %~ SANTE_PUB_INSERM %~ OPENAIRE %~ GIP-BE