%0 Journal Article %T Primary Mammary Organoid Model of Lactation and Involution %+ Plasticité cellulaire et Modélisation des Maladies / Cellular Plasticity and Disease Modelling %+ Masaryk University [Brno] (MUNI) %A Sumbal, Jakub %A Chiche, Aurélie %A Charifou, Elsa %A Koledova, Zuzana %A Li, Han %Z Work in the laboratory of HL is funded by the Pasteur, Centre National pour la Recherche the Agence Nationale de la Recherche (ANR-10-LABX-73 and ANR-16-CE13-0017-01), Fondation ARC (PJA 20161205028 and 20181208231), and AFM-Telethon Foundation. Work in the laboratory of ZK was funded by the Grant Agency of Masaryk University (grant no. MUNI/G/1446/2018). AC was funded by the postdoctoral fellowships from the Revive Consortium. JS was funded by the P-Pool (Masaryk University, Faculty of Medicine), Amgen Scholars Europe and Erasmus+ programs and by the Grant Agency of Masaryk University (grant no. MUNI/A/1565/2018). EC was funded by the Ph.D. fellowship from Sorbonne Université. %< avec comité de lecture %@ 2296-634X %J Frontiers in Cell and Developmental Biology %I Frontiers media %V 8 %P 68 %8 2020-03-19 %D 2020 %R 10.3389/fcell.2020.00068 %M 32266252 %K 3D culture %K fibroblast growth factor 2 %K involution %K lactation %K mammary gland %K milk production %K organoid %K prolactin %Z Life Sciences [q-bio]/Development Biology/MorphogenesisJournal articles %X Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involutionlike process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer. %G English %2 https://pasteur.hal.science/pasteur-03322452/document %2 https://pasteur.hal.science/pasteur-03322452/file/2020%20-%20Sumbal%20et%20al.%2C%20Front%20Dev%20Biol.pdf %L pasteur-03322452 %U https://pasteur.hal.science/pasteur-03322452 %~ PASTEUR %~ CNRS %~ ANR %~ UMR3738 %~ CELLULAR-PLASTICITY-AGE-RELATED-PATHOLOGY