%0 Journal Article %T Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes %+ Tsinghua University [Beijing] (THU) %+ Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis %+ Third Military Medical University %+ The University of Texas M.D. Anderson Cancer Center [Houston] %+ Wuhan University [China] %A Wang, Aibo %A Ding, Xiao %A Demarque, Maud %A Liu, Xindong %A Pan, Deng %A Xin, Huawei %A Zhong, Bo %A Wang, Xiaohu %A Dejean, Anne %A Jin, Wei %A Dong, Chen %Z This work was supported by National Natural Science Foundation of China Grant 041321082. %< avec comité de lecture %@ 0022-1767 %J Journal of Immunology %I Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists %V 198 %N 9 %P 3461-3470 %8 2017-04-17 %D 2017 %R 10.4049/jimmunol.1600980 %M 28314856 %Z Life Sciences [q-bio]Journal articles %X SUMOylation is an important posttranslational modification that regulates protein function in diverse biological processes. However, its role in early T cell development has not been genetically studied. UBC9 is the only E2 enzyme for all SUMOylation. In this study, by selectively deleting Ubc9 gene in T cells, we have investigated the functional roles of SUMOylation in T cell development. Loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery. Ubc9-deficient cells exhibit defective late-stage maturation post the initial positive selection with increased apoptosis and impaired proliferation, among which attenuated IL-7 signaling was correlated with the decreased survival of Ubc9-deficent CD8 single-positive cells. Furthermore, NFAT nuclear retention induced by TCR signals was regulated by SUMOylation during thymocytes development. Our study thus reveals a novel posttranslational mechanism underlying T cell development. %G English %Z We thank the flow cytometry facility of the Center for Immunology, Tsinghua University, for help in cell sorting, Dionne Prescod for help in maintaining the mouse colony, and members of Dr. Chen Dong's laboratory for technical support and assistance. %L pasteur-03234899 %U https://pasteur.hal.science/pasteur-03234899 %~ PASTEUR %~ ONO