%0 Journal Article %T Noncanonical DNA polymerization by aminoadenine-based siphoviruses %+ Génomique métabolique (UMR 8030) %+ Werkstatt fuer Potenzielle Genetik [Berlin] %+ TESSI [Paris] %+ Alderys [Orsay] %+ University of Pittsburgh (PITT) %+ Rega Institute for Medical Research [Leuven, België] %+ Chimie organique (CNRS - UMR3523) %+ Biologie des Bactéries pathogènes à Gram-positif %A Pezo, Valerie %A Jaziri, Faten %A Bourguignon, Pierre-Yves %A Louis, Dominique %A Jacobs-Sera, Deborah %A Rozenski, Jef %A Pochet, Sylvie %A Herdewijn, Piet %A Hatfull, Graham %A Kaminski, Pierre-Alexandre %A Marliere, Philippe %Z The research received funding from the European Research Council (ERC-2012-ADG-20120216/320683) and from the European Commission (ERASynBio BB/ N01023X/1 and ANR-15-SYNB-0003-04). %< avec comité de lecture %@ 0036-8075 %J Science %I American Association for the Advancement of Science (AAAS) %V 372 %N 6541 %P 520-524 %8 2021-04-29 %D 2021 %R 10.1126/science.abe6542 %M 33926956 %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biologyJournal articles %X Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3′-exonuclease but lacks 5′-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution. %G English %L pasteur-03228903 %U https://pasteur.hal.science/pasteur-03228903 %~ PASTEUR %~ CEA %~ CNRS %~ UNIV-EVRY %~ OPENAIRE %~ INC-CNRS %~ CEA-UPSAY %~ GENOMIQUE-METABOLIQUE %~ UNIV-PARIS-SACLAY %~ UNIV-EVRY-SACLAY %~ JACOB %~ CEA-DRF %~ GENOSCOPE %~ TEST-HALCNRS %~ UNIVERSITE-PARIS-SACLAY %~ ANR %~ GS-BIOSPHERA %~ GS-LIFE-SCIENCES-HEALTH %~ TEST2-HALCNRS