%0 Journal Article %T New Bacteriophages against Emerging Lineages ST23 and ST258 of Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae %+ Department of Infectious and Parasitic Diseases (University Liege) %+ Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens %+ Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven) %+ Centre of Microbial and Plant Genetics %+ Département de Microbiologie - Department of Microbiology %+ Laboratoires des Biomolécules %+ Centre de Recherche Saint-Antoine (CRSA) %+ Centre of Microbial and Plant Genetics %+ Universiteit Leiden = Leiden University %+ Centre Hospitalier René Dubos [Pontoise] %A Thiry, Damien %A Passet, Virginie %A Danis-Wlodarczyk, Katarzyna %A Lood, Cédric %A Wagemans, Jeroen %A de Sordi, Luisa %A van Noort, Vera %A Dufour, Nicolas %A Debarbieux, Laurent %A Mainil, Jacques, G %A Brisse, Sylvain %A Lavigne, Rob %Z Biermans-Lapôtre Foundation for a post-doctoral fellowship that supported D.T. work in Institut Pasteur. L.D.S. is founded by a Roux-Cantarini fellowship from the Institut Pasteur (Paris, France). R.L., K.D.-W. and J.W. are supported by a GOA grant from KU Leuven (‘Phage Biosystems’), C.L. holds a SB PhD fellowship from FWO Vlaanderen (1S64718N). K.D.-W. was supported by a PDM mandate from KU Leuven. %< avec comité de lecture %@ 1999-4915 %J Viruses %I MDPI %V 11 %N 5 %P 411 %8 2019-05-03 %D 2019 %R 10.3390/v11050411 %M 31058805 %K antimicrobial resistance %K capsule %K Galleria mellonella %K Klebsiella pneumoniae %K phage therapy %Z Life Sciences [q-bio]Journal articles %X Klebsiella pneumoniae is a bacterial pathogen of high public health importance. Its polysaccharide capsule is highly variable but only a few capsular types are associated with emerging pathogenic sublineages. The aim of this work is to isolate and characterize new lytic bacteriophages and assess their potential to control infections by the ST23 and ST258 K. pneumoniae sublineages using a Galleria mellonella larvae model. Three selected bacteriophages, targeting lineages ST258 (bacteriophages vB_KpnP_KL106-ULIP47 and vB_KpnP_KL106-ULIP54) and ST23 (bacteriophage vB_KpnP_K1-ULIP33), display specificity for capsular types KL106 and K1, respectively. These podoviruses belong to the Autographivirinae subfamily and their genomes are devoid of lysogeny or toxin-associated genes. In a G. mellonella larvae model, a mortality rate of 70% was observed upon infection by K. pneumoniae ST258 and ST23. This number was reduced to 20% upon treatment with bacteriophages at a multiplicity of infection of 10. This work increases the number of characterized bacteriophages infecting K. pneumoniae and provides information regarding genome sequence and efficacy during preclinical phage therapy against two prominent sublineages of this bacterial species. %G English %2 https://pasteur.hal.science/pasteur-03164319/document %2 https://pasteur.hal.science/pasteur-03164319/file/54%20Thiry.pdf %L pasteur-03164319 %U https://pasteur.hal.science/pasteur-03164319 %~ PASTEUR %~ APHP %~ U938 %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-MEDECINE %~ SU-MED %~ SU-TI %~ ALLIANCE-SU