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Book Sections Year : 2020

Forward Genetics in African Trypanosomes

Abstract

Forward genetic screens in Trypanosoma brucei have enabled researchers to move from a candidate-gene based approach to one where we are able to studying all genes required for a single process simultaneously. In this protocol, we describe how to generate RNAi library strains in bloodstream form trypanosomes, run a screen by selecting for drug resistance or using a reporter gene and process the high-throughput sequencing data for a genome scale RNAi library screen.
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pasteur-03107149 , version 1 (12-01-2021)

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Sebastian Hutchinson, Lucy Glover. Forward Genetics in African Trypanosomes. Trypanosomatids, 2116, pp.339-352, 2020, Methods in Molecular biology, 978-1-0716-0294-2 (online) ; 978-1-0716-0293-5 (print). ⟨10.1007/978-1-0716-0294-2_21⟩. ⟨pasteur-03107149⟩

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