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            <title xml:lang="en">New tetracycline resistance determinants coding for ribosomal protection in streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498.</title>
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                <forename type="first">D.</forename>
                <surname>Clermont</surname>
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                <forename type="first">Olivier</forename>
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            <idno type="halRefHtml">&lt;i&gt;Antimicrobial Agents and Chemotherapy&lt;/i&gt;, 1997, 41 (1), pp.112-116. &lt;a target="_blank" href="https://dx.doi.org/10.1128/AAC.41.1.112"&gt;&amp;#x27E8;10.1128/AAC.41.1.112&amp;#x27E9;&lt;/a&gt;</idno>
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                <title xml:lang="en">New tetracycline resistance determinants coding for ribosomal protection in streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498.</title>
                <author role="aut">
                  <persName>
                    <forename type="first">D.</forename>
                    <surname>Clermont</surname>
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                  <persName>
                    <forename type="first">Olivier</forename>
                    <surname>Chesneau</surname>
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                  <email type="md5">7b956501d7fcd410a3d2480ac5c0782e</email>
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                    <forename type="first">G</forename>
                    <surname>de Cespédès</surname>
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                    <forename type="first">T</forename>
                    <surname>Horaud</surname>
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                <idno type="eissn">1098-6596</idno>
                <title level="j">Antimicrobial Agents and Chemotherapy</title>
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                  <publisher>American Society for Microbiology</publisher>
                  <biblScope unit="volume">41</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">112-116</biblScope>
                  <date type="datePub">1997-01-01</date>
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              <classCode scheme="mesh">Amino Acid Sequence</classCode>
              <classCode scheme="mesh">Bacterial Proteins</classCode>
              <classCode scheme="mesh">Polymerase Chain Reaction</classCode>
              <classCode scheme="mesh">Ribosomes</classCode>
              <classCode scheme="mesh">Streptococcus pyogenes</classCode>
              <classCode scheme="mesh">Tetracycline Resistance</classCode>
              <classCode scheme="mesh">Base Sequence</classCode>
              <classCode scheme="mesh">Cloning, Molecular</classCode>
              <classCode scheme="mesh">Evolution, Molecular</classCode>
              <classCode scheme="mesh">GTP-Binding Proteins</classCode>
              <classCode scheme="mesh">Gene Amplification</classCode>
              <classCode scheme="mesh">Genes, Bacterial</classCode>
              <classCode scheme="mesh">Minocycline</classCode>
              <classCode scheme="mesh">Molecular Sequence Data</classCode>
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            <abstract xml:lang="en">
              <p>An approach based on PCR has been developed to identify new members of the tet gene family in streptococci resistant to tetracycline and minocycline. Degenerate primers, corresponding to portions of the conserved domains of the proteins Tet(M), Tet(O), TeTB(P), Tet(Q), and Tet(S), all specifying the tetracycline-minocycline resistance phenotype, were used to selectively amplify DNA fragments within the coding sequences. Nine streptococcal strains which do not carry the genes tet(M), tet(O), tetB(P), tet(Q), or tet(S) were investigated. Four of them gave no detectable PCR products. The five remaining strains each yielded a PCR product of 1.1 kbp. DNA hybridization experiments showed that these putative Tet determinants fell into four new hybridization classes, of which one, Tet T, was further analyzed. The gene tet(T) was isolated from Streptococcus pyogenes A498, and the nucleotide sequence that was necessary and sufficient for the expression of tetracycline resistance in Escherichia coli was determined. The deduced Tet(T) protein consists of 651 amino acids. The protein most closely related to Tet(T) was Tet(Q), which has 49% identical amino acid residues. A phylogenetic analysis revealed that Tet T represents a novel branching order among the Tet determinants so far described.</p>
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              <org type="consortium">We thank Ne vine El Solh and Karen Pepper for criticism of themanuscript and Catherine Tran for secretarial assistance.We are grateful to Patrice Courvalin for giving us pAT101, pAT451,and pAT456, Diane Taylor for pUOA4, Julian Rood for pJIR939, and Jean-Michel Lacroix for pBSK1.2-5</org>
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