%0 Journal Article %T Anti-Inflammatory Role for Intracellular Dimeric Immunoglobulin A by Neutralization of Lipopolysaccharide in Epithelial Cells %+ Pathogénie Microbienne Moléculaire %+ Analyse d'images biologiques - Biological Image Analysis (BIA) %A Fernandez, M.Isabel %A Pedron, Thierry %A Tournebize, Régis, R. %A Olivo-Marin, Jean-Christophe %A Sansonetti, Philippe %A Phalipon, Armelle %Z P.J.S. is a Howard Hughes Medical Institute Scholar. M.I.F. is supported by the Institut Pasteur (Bourse Cantarini) and the Fondation pour la Recherche Médicale. %< avec comité de lecture %@ 1074-7613 %J Immunity %I Elsevier %V 18 %N 6 %P 739-749 %8 2003-05-13 %D 2003 %R 10.1016/S1074-7613(03)00122-5 %M 12818156 %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Intestinal epithelial cells (IEC) play a central role in innate and acquired mucosal immunity. They ensure early signaling to trigger an inflammatory response against pathogens. Moreover, IEC mediate transcytosis of dimeric IgA (dIgA), through the polymeric-immunoglobulin receptor (pIgR), to provide secretory IgA, the major protective Ig in mucosal secretions. Using an in vitro model of polarized IEC, we describe an additional anti-inflammatory mechanism of dIgA-mediated protection against intracellular bacterial components involved in the proinflammatory activation of IEC. Specific dIgA colocalizes to lipopolysaccharide (LPS) in the apical recycling endosome compartment, preventing LPS-induced NF-kappaB translocation and subsequent proinflammatory response. Thus, intracellular neutralization by dIgA limits the acute local inflammation induced by proinflammatory pathogen-associated molecular patterns such as LPS. %G English %Z We are grateful to M. Mavris for careful reading of the manuscript and helpful discussion and B. Corthésy and J.P Kraehenbuhl for their kind advice. %L pasteur-02875032 %U https://pasteur.hal.science/pasteur-02875032 %~ PASTEUR %~ CNRS %~ CDF %~ FRM %~ BIOLOGICAL-IMAGE-ANALYSIS %~ UMR3691