%0 Journal Article %T Condensin-Mediated Chromosome Folding and Internal Telomeres Drive Dicentric Severing by Cytokinesis %+ Stabilité génétique, cellules souches et radiations (SGCSR (U_1274 / UMR_E_008)) %+ Régulation spatiale des Génomes - Spatial Regulation of Genomes %A Guerin, Thomas %A Béneut, Claire %A Barinova, Natalja %A López, Virginia %A Lazar-Stefanita, Luciana %A Deshayes, Alice %A Thierry, Agnès %A Koszul, Romain %A Dubrana, Karine %A Marcand, Stephane %Z This work was supported by funding to S.M. from Fondation ARC, EDF, CEA Radiobiology call, DRF-Impulsion (4D-DSB-DIC), and ANR (DICENs-ANR-14-CE10-0021-01); to K.D. from the European Research Council under the Seventh Framework Program (FP7/2007 2013/ERC grant agreement 281287); and to R.K. from the European Research Council under the Horizon 2020 Program (ERC grant agreement 260822). T.M.G. was supported by a PhD fellowship from CEA, ANR, and a Fondation ARC young researcher grant. %< avec comité de lecture %@ 1097-2765 %J Molecular Cell %I Cell Press %V 75 %N 1 %P 131-144.e3 %8 2019-07 %D 2019 %R 10.1016/j.molcel.2019.05.021 %M 31204167 %K telophase %K yeast %K mutagenesis %K Hi-C %K SMC %K Telomere %K abscission %K condensing %K lacI %K mitosis %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biologyJournal articles %X In Saccharomyces cerevisiae, dicentric chromosomes stemming from telomere fusions preferentially break at the fusion. This process restores a normal karyotype and protects chromosomes from the detrimental consequences of accidental fusions. Here, we address the molecular basis of this rescue pathway. We observe that tandem arrays tightly bound by the telomere factor Rap1 or a heterologous high-affinity DNA binding factor are sufficient to establish breakage hotspots, mimicking telomere fusions within dicentrics. We also show that condensins generate forces sufficient to rapidly refold dicentrics prior to breakage by cytokinesis and are essential to the preferential breakage at telomere fusions. Thus, the rescue of fused telomeres results from a condensin- and Rap1-driven chromosome folding that favors fusion entrapment where abscission takes place. Because a close spacing between the DNA-bound Rap1 molecules is essential to this process, Rap1 may act by stalling condensins. %G English %Z We thank Angela Taddei for lacI, lacI∗∗, and lacO array plasmids and suggestions; Frank Uhlmann and Thomas Kuilman for the G20 plasmid; Helle Ulrich for the AID tool kit; Didier Busso and Eléa Dizet (CIGEX platform) for the Rap1 sites plasmids; Pascale Lesage for the anti-Dps1 antibody; Rémi Montagne for assistance with the Hi-C data; Romain Le Bars (IMAGE-GIF platform) and Lamya Irbah (IRCM microscopy platform) for assistance with higher-resolution microscopy; Dan Throsby for text editing; and John Marko, Damien D’Amours, Sarah Lambert, François-Xavier Barre, Pablo Radicella, Eric Coïc, Laurent Maloisel, Paul-Henri Roméo, Mathias Toulouze, and Maoussi Lhuillier-Akakpo for fruitful discussions and suggestions. %2 https://pasteur.hal.science/pasteur-02866762/document %2 https://pasteur.hal.science/pasteur-02866762/file/S1097276519303934.pdf %L pasteur-02866762 %U https://pasteur.hal.science/pasteur-02866762 %~ PASTEUR %~ CEA %~ CNRS %~ OPENAIRE %~ DSV %~ CEA-UPSAY %~ UNIV-PARIS-SACLAY %~ CEA-UPSAY-SACLAY %~ CEA-DRF %~ UP-SCIENCES %~ TEST-HALCNRS %~ ANR %~ ELSEVIER %~ UMR-SGCSR %~ SPATIAL-REGULATION-GENOMES %~ UMR3525