%0 Journal Article %T Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells %+ Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens (BBPG+) %+ Barrières et Pathogènes %+ Centre national de Référence des Streptocoques (CNR) %A Périchon, Bruno %A Guignot, Julie %A Szili, Noémi %A Gao, Cherry %A Poyart, Claire %A Trieu-Cuot, Patrick %A Dramsi, Shaynoor %Z This work was supported by the DIM Malinf from the Conseil Régional d’Ile-de-France (Grant DIM130065) to CP and SD and the French Government's Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” Grant ANR-10-LABX-62-IBEID. NS was recipient of a doctoral fellowship from the DIM Malinf. %< avec comité de lecture %@ 1286-4579 %J Microbes and Infection %I Elsevier %V 21 %N 2 %P 99-103 %8 2019-03 %D 2019 %R 10.1016/j.micinf.2018.10.004 %M 30419351 %K THP-1 phagocytosis %K PI-2b pilus %K GBS %K S. agalactiae %K Group B Streptococcus %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages. %G English %2 https://pasteur.hal.science/pasteur-02863685/document %2 https://pasteur.hal.science/pasteur-02863685/file/1-s2.0-S1286457918301667-main.pdf %L pasteur-02863685 %U https://pasteur.hal.science/pasteur-02863685 %~ PASTEUR %~ UNIV-PARIS5 %~ CNRS %~ APHP %~ USPC %~ UNIV-PARIS %~ UP-SANTE %~ ANR