%0 Journal Article %T Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury %+ Physiopathologie et Thérapeutiques Respiratoires %+ Défense innée et inflammation %+ CHU Saint-Antoine [AP-HP] %+ Réanimation Médicale [CHU Henri Mondor - APHP] (DHU A-TVB) %+ Service de physiologie, explorations fonctionnelles [Mondor] %A Attalah, H.L. %A Wu, Yongzheng %A Alaoui-El-Azher, M. %A Thouron, F. %A Koumanov, K. %A Wolf, C. %A Brochard, L. %A Harf, A. %A Delclaux, C. %A Touqui, L. %< avec comité de lecture %@ 0903-1936 %J European Respiratory Journal %I European Respiratory Society %V 21 %N 6 %P 1040-1045 %8 2003-06-01 %D 2003 %R 10.1183/09031936.03.00093002 %M 12797501 %K alpha naphthylthiourea %K compliance %K endotoxin %K Pseudomonas aeruginosa %Z Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tractJournal articles %X The aim of this study was to evaluate the presence of type-II secretory phospholipase A2 (sPLA2-IIA) in alveolar space and its possible role in the destruction of surfactant in three rat models of acute lung injury. Alveolar instillation of either lipopolysaccaride or live Pseudomonas aeruginosa resulted in a significant increase in lung oedema and in a decrease in static compliance of the respiratory system together with alveolar-neutrophil influx as compared with healthy control rats. The upregulation of messenger ribonucleic acid and sPLA2-IIA by the lung was evident. This was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in bacteria-instilled rats. A negative correlation between compliance and sPLA2-IIA activity in bronchoalveolar lavage fluid was shown. By contrast, during alpha naphthylthiourea-induced injury, neither alveolar-neutrophil influx nor increase in sPLA2-IIA activity was observed. Additional experiments in rats treated with a specific inhibitor of type-II secretory phospholipase A2 activity (3 acetamine-1-benzyl-2 ethylindolyl-5 oxy; propane phosphonic acid (LY311727)) demonstrated no improvement in physiological parameters despite a biochemical effect, suggesting that its activity is only one of the multiple factors involved in the pathophysiology of lung injury. %G English %Z The authors would like to thank H. Arita (Shionogi Research laboratory, Osaka, Japan) for the generous gift of the rat sPLA2-IIA cDNA. %L pasteur-02863076 %U https://pasteur.hal.science/pasteur-02863076 %~ INSERM %~ PASTEUR %~ APHP %~ UPEC %~ SORBONNE-UNIVERSITE %~ SU-INF-2018 %~ SU-MEDECINE %~ SU-MED %~ SU-TI %~ ALLIANCE-SU