%0 Journal Article %T The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells %+ Department of Histology and Embryology [Brno] (MED / MUNI) %+ Central European Institute of Technology [Brno] (CEITEC MU) %+ Institute of Biophysics of the Czech Academy of Sciences (IBP / CAS) %+ Hub d'analyse d'images - Image Analysis Hub (Platform) (IAH) %+ St. Anne’s University Hospital [Brno] %A Pospíšilová, Veronika %A Ešner, Milan %A Červenková, Iveta %A Fedr, Radek %A Tinevez, Jean-Yves %A Hampl, Aleš %A Anger, Martin %Z This work was supported by Czech Science Foundation projects 15-11707S and 15-04844S andthe European Regional Development Fund-Project “National infrastructure for biological and medical imaging” (No.CZ.02.1.01/0.0/0.0/16_013/000 1775). %< avec comité de lecture %@ 1214-021X %J Journal of Applied Biomedicine %I University of South Bohemia in České Budějovice %V 17 %N 4 %P 209-217 %8 2019-12-13 %D 2019 %R 10.32725/jab.2019.018 %K Mitosis length %K Multipolar division %K Embryonic stem (ES) cells %K Single-cell tracking %K Spindle assembly checkpoint (SAC) %K Time-lapse microscopy %Z Life Sciences [q-bio]Journal articles %X Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability. %G English %Z We thank Drs. Anthony Hyman and Ina Poser (MPICBG, Dresden, Germany) for providing the H2A-GFP BAC construct, Dr. Ronald Naumann (MPI-CBG, Dresden, Germany) for providing the JM8.A mouse ES cells, and Dr. Klara Koudelkova for her excellent technical assistance %2 https://pasteur.hal.science/pasteur-02616481/document %2 https://pasteur.hal.science/pasteur-02616481/file/The%20frequency%20and%20consequences%20of%20multipolar%20mitoses%20in%20undifferentiated%20embryonic%20stem%20cells.pdf %L pasteur-02616481 %U https://pasteur.hal.science/pasteur-02616481 %~ PASTEUR