%0 Journal Article %T A packing for A-form DNA in an icosahedral virus %+ University of Virginia %+ Virologie des archées - Archaeal Virology %+ Département de Microbiologie - Department of Microbiology %+ University of Pittsburgh School of Medicine %+ Royal Netherlands Institute for Sea Research (NIOZ) %+ Utrecht University [Utrecht] %+ Ivane Javakhishvili Tbilisi State University (TSU) %A Wang, Fengbin %A Liu, Ying %A Su, Zhangli %A Osinski, Tomasz %A de Oliveira, Guilherme A. P. %A Conway, James F. %A Schouten, Stefan %A Krupovic, Mart %A Prangishvili, David %A Egelman, Edward H %Z This work was supported by NIH Grant GM122510 (to E.H.E.); the European Union’s Horizon 2020 Research and Innovation Program Grant 685778, project VIRUS X (to D.P.); and l’Agence Nationale de la Recherche Grant ANR-17-CE15-0005-01 (to M.K.). Imaging by the National Cancer Institute (NCI) National Cryo-EM Facility (Frederick National Laboratory for Cancer Research) was supported by contract HSSN261200800001E. %Z The data reported in this paper have been deposited in the Electron Microscopy Data Bank, https://www.ebi.ac.uk/pdbe/emdb/ (accession no. 20083) and in the Protein Data Bank (ID 6OJ0). %< avec comité de lecture %@ 0027-8424 %J Proceedings of the National Academy of Sciences of the United States of America %I National Academy of Sciences %V 116 %N 45 %P 22591 - 22597 %8 2019-11-05 %D 2019 %R 10.1073/pnas.1908242116 %M 31636205 %K DNA structure %K cryo-EM %K hyperthermophilic acidophiles %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-Å resolution the structure of the Sulfolobus polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a T = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus. %G English %Z We thank Dr. Ulrich Baxa and Dr. Thomas Edwards for their help in NCI data collection; the Ultrastructural BioImaging unit of Institut Pasteur for access to electron microscopes; and Dr. Wen Jiang (Purdue University) for very helpful discussions. %L pasteur-02559906 %U https://pasteur.hal.science/pasteur-02559906 %~ PASTEUR %~ ANR