The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis - Institut Pasteur
Article Dans Une Revue Nature Communications Année : 2020

The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis

Audrey Salles

Résumé

Cytokinesis requires the constriction of ESCRT-III filaments on the side of the midbody, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site. To reveal actors involved in abscission, we obtained the proteome of intact, post-abscission midbodies (Flemmingsome) and identified 489 proteins enriched in this organelle. Among these proteins, we further characterized a plasma membrane-to-ESCRT module composed of the transmembrane proteoglycan syndecan-4, ALIX and syntenin, a protein that bridges ESCRT-III/ALIX to syndecans. The three proteins are highly recruited first at the midbody then at the abscission site, and their depletion delays abscission. Mechanistically, direct interactions between ALIX, syntenin and syndecan-4 are essential for proper enrichment of the ESCRT-III machinery at the abscission site, but not at the midbody. We propose that the ESCRT-III machinery must be physically coupled to a membrane protein at the cytokinetic abscission site for efficient scission, uncovering common requirements in cytokinesis, exosome formation and HIV budding.

Mots clés

Fichier principal
Vignette du fichier
Flemmingsome reveals an ESCRT-to-membrane coupling.pdf (3.73 Mo) Télécharger le fichier
Origine Publication financée par une institution
Loading...

Dates et versions

pasteur-02557366 , version 1 (28-04-2020)

Licence

Identifiants

Citer

Cyril Addi, Adrien Presle, Stéphane Frémont, Frédérique Cuvelier, Murielle Rocancourt, et al.. The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis. Nature Communications, 2020, 11 (1), pp.1941. ⟨10.1038/s41467-020-15205-z⟩. ⟨pasteur-02557366⟩
293 Consultations
160 Téléchargements

Altmetric

Partager

More