%0 Journal Article %T Comparative analysis of virus–host interactomes with a mammalian high-throughput protein complementation assay based on Gaussia princeps luciferase %+ Génétique, Papillomavirus et Cancer Humain %+ Génomique Virale et Vaccination %+ Logiciels et Banques de Données %+ IFR128 %+ Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique %+ Center for Cancer Systems Biology (CCSB) %+ Harvard Medical School [Boston] (HMS) %A Neveu, Grégory %A Cassonnet, Patricia %A Vidalain, Pierre-Olivier %A Rolloy, Caroline %A Mendoza, José %A Jones, Louis %A Tangy, Frédéric %A Muller, Mandy %A Demeret, Caroline %A Tafforeau, Lionel %A Lotteau, Vincent %A Rabourdin-Combe, Chantal %A Travé, Gilles %A Dricot, Amélie %A E.Hill, David %A Vidal, Marc %A Favre, Michel %A Jacob, Yves %Z This work was supported in part by funding from the Institut Pasteur, and by grants from the Ligue nationale contre le Cancer (grants R05/75-129 and RS07/75-75), the Association pour la Recherche sur le Cancer (grants 3731XA0531F and 4867), and the Agence Nationale de la Recherche (EPI-HPV-3D program) and by Center of Excellence in Genomic Science (CEGS) grants P50-HG004233 and R01-H6001715 from the National Human Genome Research Institute (NHGRI) (M. V, DE.H). G.N. was a recipient of the Conseil Général de la Vienne and Pasteur Weizmann foundation. C.R. was a recipient of a Ministère de l’éducation nationale, de la recherche et de la technologie (MENRT) fellowship. %< avec comité de lecture %@ 1046-2023 %J Methods %I Elsevier %V 58 %N 4 %P 349-359 %8 2012-12 %D 2012 %R 10.1016/j.ymeth.2012.07.029 %M 22898364 %K Comparative interactomics %K HPV %K E6 %K E7 %K Interactome %K Complementation assay %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X Comparative interactomics is a strategy for inferring potential interactions among orthologous proteins or ''interologs''. Herein we focus, in contrast to standard homology-based inference, on the divergence of protein interaction profiles among closely related organisms, showing that the approach can correlate specific traits to phenotypic differences. As a model, this new comparative interactomic approach was applied at a large scale to human papillomaviruses (HPVs) proteins. The oncogenic potential of HPVs is mainly determined by the E6 and E7 early proteins. We have mapped and overlapped the virus-host protein interaction networks of E6 and E7 proteins from 11 distinct HPV genotypes, selected for their different tropisms and pathologies. We generated robust and comprehensive datasets by combining two orthogonal protein interaction assays: yeast two-hybrid (Y2H), and our recently described ''high-throughput Gaussia princeps protein complementation assay'' (HT-GPCA). HT-GPCA detects protein interaction by measuring the interaction-mediated reconstitution of activity of a split G. princeps luciferase. Hierarchical clustering of interaction profiles recapitulated HPV phylogeny and was used to correlate specific virus-host interaction profiles with pathological traits, reflecting the distinct carcinogenic potentials of different HPVs. This comparative interactomics constitutes a reliable and powerful strategy to decipher molecular relationships in virtually any combination of microorganism-host interactions. %G English %Z We thank Richard Paul, Katherine Kean and Michael Cusick for critical reading of the manuscript %L pasteur-01971543 %U https://pasteur.hal.science/pasteur-01971543 %~ PASTEUR %~ CNRS %~ UNIV-STRASBG1 %~ UNIV-STRASBG %~ SITE-ALSACE %~ ANR