%0 Journal Article %T Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms %+ Cellules Souches et Développement / Stem Cells and Development %+ Cellule Pasteur UPMC %A Perez-Mockus, Gantas %A Roca, Vanessa %A Mazouni, Khalil %A Schweisguth, François %Z This work was funded by an Agence Nationale de la Recherche grant (ANR12-BSV2-0010-01). G. Perez-Mockus received fellowships from the Ministére de l’Education Nationale de la Recherche et de la Technologie and the Fondation pour la Recherche Médicale. %< avec comité de lecture %@ 0021-9525 %J Journal of Cell Biology %I Rockefeller University Press %V 216 %N 5 %P 1405 - 1420 %8 2017-05-01 %D 2017 %R 10.1083/jcb.201611196 %M 28400441 %Z Life Sciences [q-bio]/Development BiologyJournal articles %X Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we identify the Crb complex protein Stardust (Sdt) as a target of the E3 ubiquitin ligase Neuralized (Neur) in Drosophila melanogaster. Neur interacts with and down-regulates specific Sdt isoforms containing a Neur binding motif (NBM). Using a CRISPR (clustered regularly interspaced short palindromic repeats)-induced deletion of the NBM-encoding exon, we found that Sdt is a key Neur target and that Neur acts via Sdt to down-regulate Crb. We further show that Neur promotes the endocytosis of Crb via the NBM-containing isoforms of Sdt. Although the regulation of Crb by Neur is not strictly essential, it contributes to epithelium remodeling in the posterior midgut and thereby facilitates the trans-epithelial migration of the primordial germ cells in early embryos. Thus, our study uncovers a novel regulatory mechanism for the developmental control of Crb-mediated morphogenesis. %G English %2 https://pasteur.hal.science/pasteur-01799354/document %2 https://pasteur.hal.science/pasteur-01799354/file/1405.full.pdf %L pasteur-01799354 %U https://pasteur.hal.science/pasteur-01799354 %~ PASTEUR %~ UPMC %~ CNRS %~ SORBONNE-UNIVERSITE %~ SU-MEDECINE %~ SU-MED %~ SU-TI %~ ANR %~ ALLIANCE-SU %~ FRM %~ UMR3738 %~ STEM-CELLS-DEVELOPMENT