%0 Journal Article %T Enterococcus faecalis promotes innate immune suppression and polymicrobial catheter-associated urinary tract infection %+ Nanyang Technological University [Singapour] %+ Agency for science, technology and research [Singapore] (A*STAR) %+ Immunobiologie des Cellules dendritiques %A Tien, Brenda Yin Qi %A Goh, Hwee Mian Sharon %A Chong, Kelvin Kian Long %A Bhaduri-Tagore, Soumili %A Holec, Sarah %A Dress, Regine %A Ginhoux, Florent %A Ingersoll, Molly A %A Williams, Rohan B H %A Kline, Kimberly A %Z BYQT, HMSG, KKLC, SBT, SH, and KAK were supported by the National Research Foundation under its Singapore NRF Fellowship programme (NRF-NRFF2011-11) and by the Ministry of Education Singapore under its Tier 2 programme (MOE2014-T2-1-129). FG and RD were supported by Singapore Immunology Network core funding, Agency for Science, Technology and Research (A*STAR), Singapore. %< avec comité de lecture %@ 0019-9567 %J Infection and Immunity %I American Society for Microbiology %V 85 %N 12 %P e00378-17 %8 2017-11 %D 2017 %R 10.1128/IAI.00378-17 %M 28893918 %Z Life Sciences [q-bio]/ImmunologyJournal articles %X Enterococcus faecalis, a member of the human gastrointestinal microbiota, is an opportunistic pathogen associated with hospital-acquired wound, bloodstream, and urinary tract infections. E. faecalis can subvert or evade immune-mediated clearance, although the mechanisms are poorly understood. In this study, we examined E. faecalis-mediated subversion of macrophage activation. We observed that E. faecalis actively prevents NF-κB signaling in mouse RAW264.7 macrophages in the presence of Toll-like receptor agonists and during polymicrobial infection with Escherichia coliE. faecalis and E. coli co-infection in a mouse model of catheter-associated urinary tract infection (CAUTI) resulted in a suppressed macrophage transcriptional response in the bladder compared to E. coli infection alone. Finally, we demonstrated that co-inoculation of E. faecalis with a commensal strain of E. coli into catheterized bladders significantly augmented E. coli CAUTI. Taken together, these results support that E. faecalis suppression of NF-κB-driven responses in macrophages promotes polymicrobial CAUTI pathogenesis especially when co-infected with less virulent or commensal E. coli strains. %G English %Z We thank Krithika Arumugam for her assistance with sequence data processing. We also thank Gary Dunny and Jennifer Dale for providing the E.faecalis transposon mutants used in this study. This work benefitted from data assembled by the ImmGen Consortium (41). BYQT, HMSG, KKLC, SBTSH, RBHW, and KAK were supported by the National Research Foundation and Ministry of Education Singapore under its Research Centre of Excellence Programme %L pasteur-01620314 %U https://pasteur.hal.science/pasteur-01620314 %~ PASTEUR