%0 Journal Article %T TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures. %+ Signalisation Moléculaire et Activation Cellulaire (SMAC) %+ Imagerie Dynamique (Plate-Forme) (PFID) %+ Trafic membranaire et Division cellulaire %+ Rockefeller University [New York] %+ Imagerie et Modélisation %+ Instituto de Medicina Molecular (iMM) %+ University College of London [London] (UCL) %+ Council for Scientific and Industrial Research [Pretoria] (CSIR) %A Tarantino, Nadine %A Tinevez, Jean-Yves %A Crowell, Elizabeth Faris %A Boisson, Bertrand %A Henriques, Ricardo %A Mhlanga, Musa %A Agou, Fabrice %A Israël, Alain %A Laplantine, Emmanuel %Z This work was supported by grants from the “Fondation ARC pour la Recherche sur le Cancer” (No. SFI20121205641) and the “Ligue Contre le Cancer” to E. Laplantine. %< avec comité de lecture %@ 0021-9525 %J Journal of Cell Biology %I Rockefeller University Press %V 204 %N 2 %P 231-45 %8 2014-01-20 %D 2014 %R 10.1083/jcb.201307172 %M 24446482 %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]Journal articles %X Nuclear factor κB (NF-κB) essential modulator (NEMO), a regulatory component of the IκB kinase (IKK) complex, controls NF-κB activation through its interaction with ubiquitin chains. We show here that stimulation with interleukin-1 (IL-1) and TNF induces a rapid and transient recruitment of NEMO into punctate structures that are anchored at the cell periphery. These structures are enriched in activated IKK kinases and ubiquitinated NEMO molecules, which suggests that they serve as organizing centers for the activation of NF-κB. These NEMO-containing structures colocalize with activated TNF receptors but not with activated IL-1 receptors. We investigated the involvement of nondegradative ubiquitination in the formation of these structures, using cells deficient in K63 ubiquitin chains or linear ubiquitin chain assembly complex (LUBAC)-mediated linear ubiquitination. Our results indicate that, unlike TNF, IL-1 requires K63-linked and linear ubiquitin chains to recruit NEMO into higher-order complexes. Thus, different mechanisms are involved in the recruitment of NEMO into supramolecular complexes, which appear to be essential for NF-κB activation. %G English %2 https://pasteur.hal.science/pasteur-01539965/document %2 https://pasteur.hal.science/pasteur-01539965/file/231.full.pdf %L pasteur-01539965 %U https://pasteur.hal.science/pasteur-01539965 %~ PASTEUR %~ CNRS